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-Cells are the basic units of organization and live in all living organisms -All cells come from pre-existing cells -All living cells evolved from common ancestor |
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Consists of the lipid bilayer embedded with protein |
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-Forms from phospholips with water (due to phosphate head is hydrophilic and fatty acid tail is hydrophobic) -Pass through: hydrophobic molecules, water. Not: ions |
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-Magnification: 1500-2000x -Resolution: 0.2 um -Dead or alive cells -Orcein (protein) stains |
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-Magnification: 1,000,000c -Resolution- 1um - 0.2um -Dead cells only -Sections/Surface cell |
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Biochemical Technique to Study Cells |
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-Centrifuge: puts certain parts into pellets after time durations (nuclei, mitochondria, in small pellet : ER, Golgi, plasma membrane) |
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Prokaryotic Cell (example, size, characteristics, compnonents) |
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-Examples: Domain Bacteria and Archaea -Size: 1-10um -Lacks nucleus and membrane-bound organelles -Cell wall: made of peptidoglycan (polymer of sugars and amino acids) |
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Eukaryotic Cells (example, size, characteristics, compnonents) |
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-Example: Domain Eukarya -Size range: 10 - 100 um -Contains Nucleus and other membrane-bound organelles |
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System network of organelles involved in manufacturing/transporting materials/proteins -nucleus, endoplasmic reticulum (ER), Golgi complex, lysosomes, vesicles, and vacuoles. |
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-Most prominent organelle, "cell brain" -Surrounded by nuclear envelope with nuclear pores in it -Has a nucleolus: site of ribose assembly -Has chromatin/chromosomes |
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Nuclear Envelope/Pores Characterisits |
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Nuclear envelope is double membrane -Pores regulate the passage of materials between nucleoplasm and cytoplasm |
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-Inside the nucleus -Duplicates through Replication -Organized into units called genes |
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-DNA+histone proteins -Condenses into chromosomes |
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-Composed of membranes and lumen -manufactures, processes, and transports a wide variety of biochemical compounds for use inside and outside of the cell. |
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-Synthesizes lipids and carbohydrates. -In liver, enzymes help detoxify it -An example: breaks down alcohol |
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-Membrane is bound with ribosomes -Synthesis and manufacture proteins -In pancreatic cells to help pancreas function/digest |
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Aquatic environment within a cell. |
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aka Cell membrane: biological membrane that separates the interior of all cells from the outside environment |
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Composed of rRNA (ribosomal RNA) + proteins -Synthesized in the nucleolus -Two types: free in the cytoplasm and membrane-bound ribosomes -Fuction: protein synthesis |
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-Components: membrane sacs called cisternae (cis face, trans face, media region). -Receives materials from transport vesicles which bring molecules from the ER. -Function: protein sorter and modification |
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-Small vesicles with digestive enzymes -Two kinds: primary and secondary lysosomes -Function rid of waste materials |
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Primary + Secondary Lysosomes |
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-Bud from golgi apparatus -Synthesized in rough ER -They swallow outside material to turn into secondary lysosomes |
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Examples of Lysosome malfunctions |
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Rheumatid Arthritis: Leaky Lysosomes Tay-Sachs disease: mutation, hydrolytic enzyme is not functional |
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-Vesicles holding digestive enzymes -Absorb nutrients and digest fatty acids and alcohol |
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Specialized peroxisomes in oily seeds (e.g. peanuts) |
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-Water filled storage spaces in cells -store food, collect waste products, and can help with cell shape and buoyancy |
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-Surrounded by membrane called tonoplast -90% of cell volume -When they grow, turgor pressure: push plasma membrane against cell wall |
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-In plant cells only: acts as pressure vessel/prevents overexpansion when cells fillw ith water
Consists of: -Primary cell wall: cellulose -Secondary cell wall: lignin -In the middle lamella in primary cell wall: pectin (helps bind cells together) |
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Functions: aerobic respiration: converts energy into ATP (oxygen needed) and lets out waste -Membrane bound and in most eukaryotic cells -Not part of endomembrane system |
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-Only found in plant cells/not part of endomembrane system
-Helps process of photosynthesis by storing/capturing sunlight to produce glucose (food for the plant) |
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-Protein fibers that protect against wear and tear -Microfilaments (muscle contractions), microtubules(determine cell shapes and form the spindle fibers which separate chromosomes during mitosis) and intermediate filaments (more strength) |
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-Whip-like appendages only on surface of eukaryotic cells and animal cells -Function: move liquids past surface of the cells |
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Cilia and Flagella Differences |
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Cilia is hair-like, short and many Flagella more whip-like and few. |
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Extracellular Matrix (ECM) |
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Network of non-living tissue that supports cell -Have fibronectins (glycoproteins of the ECM) that bind to integrin and other receptors in the plasma membrane |
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It transfers and carries genetic material from the DNA (in the nucleus) to the ribosome/cytoplasm for the manufacture of protein. |
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