Term
| Malaria is caused by what? |
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Definition
| blood borne disease caused by Plasmodium falciparum, spread by mosquito bites |
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Term
| Where does one stage of the Plasmodium life cycle occur? |
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Definition
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Term
| Draw the three main stages of malaria |
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Definition
| The mosquito stage, the human liver stage, and the human blood stage |
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Term
| What does the Michaelis Menten equation describe? |
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Definition
| The kinetic behavior of many enzymes |
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Term
| The rate of an enzyme catalyzed reaction is most dependent on substrates on what concentration? |
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Definition
| Low substrate concentrations |
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Term
| What does a treatment of urea and beta-mercaptoethanol do to a ribonuclease structure? |
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Definition
| It denatures the protein, but only disrupts the secondary and tertiary structure, not the primary |
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Term
| What would be the rate if [S] is equal to Km? |
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Definition
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Term
| What must you know in order to determine Km and Vmax? |
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Definition
| substrate concentration and Michaelis constant |
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Term
| The hemoglobin HbS mutation leads to what? |
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Definition
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Term
| What is the treatment for acetylcholinesterase inhibition? |
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Definition
| Atropine, which blocks the binding of acetylcholine to its receptor. |
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Term
| What does the inhibition of acetylcholinesterase cause? |
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Definition
| Acetylcholine is permanently bound to its receptor and results in prolonged nerve firing. |
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Term
| What happens with an irreversible enzyme inhibitor? |
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Definition
| The enzyme is covalently modified; therefore, no longer a catalyst. |
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Term
| What do organofluorophosphates do? |
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Definition
| Form a covalent product with the active site of an enzyme(serine), which inactivates the enzyme. |
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Term
| What happens during competitive inhibition? |
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Definition
| The inhibitor mimics the substrate, Km increases, Vmax stays the same. |
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Term
| Draw a Lineweaver Burk plot that shows the presence and absence of a competitive inhibitor. |
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Definition
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Term
| What is the velocity of an equation dependent on? |
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Definition
|
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Term
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Definition
| the reaction rate V (velocity) at a given substrate concentration ([S]) |
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Term
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Definition
| Maximum velocity of a reaction |
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Term
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Definition
| the Michaelis constant; dependent on the affinity of the enzyme for its substrate |
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Term
| How do you find Km on a graph? |
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Definition
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Term
| Why is the malaria resisting phenotype semi-dominant? |
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Definition
| The HbS mutation in the heterozygous state causes the blood cell to sickle and lyse when infected with plasmodium. |
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Term
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Definition
| helps with the diffusion of O2 in the muscle tissue |
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Term
| What does hemoglobin do in regards to O2? |
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Definition
| binds oxygen in the lungs and transports it to other tissues |
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Term
| When the electron shell of Fe condenses, what proximal structures move in close to the heme plan? |
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Definition
| histone residue and F-helix |
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Term
| Hemoglobin exhibits what type of binding, and what does it mean? |
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Definition
| Cooperative binding, where the binding of oxygen to one monomer increases the affinity for oxygen for the other monomer. |
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