Term
| What are the phases and associated temperatures used in antibody id |
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Definition
immediate spin- no temp detected
37.C albumin/ LISS- 37.C
AHG- 37.C |
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Term
| what is the priniciple of the antibody id procedure |
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Definition
| establish a pattern of reactivity; pattern established by the series of positive/negative reactions that are seen when testing the serum with the panel cells. antibody id is most often concerned w/ non- ABO antibodies that may appear in a patients serum |
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Term
| how do you read reactions for antibody id |
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Definition
reactions are read macroscopically for aggulation and/or hemolysis.
all positive reactions must be graded for their strength |
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Term
| serological properties (antibody id) |
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Definition
| phase /temp reactions; strength of reaction; presence or absence of hemolysis reactions; lewis system antibodies can react at all phases and temps |
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Term
| specific specimen requirement and collection procedures |
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Definition
| fresh within 72 hrs of drawing if refrigerated; non-hemolyzed; red- top collection tube ( serum separators may bind antibodies) |
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Term
| factors that cause inaccurate antibody id results |
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Definition
rouleaux seen in saline/albumin phases not in AHG phase.
solve by using saline replacement technique |
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Term
| quality control requirements for antibody id |
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Definition
consists of a mixture of the persons own red cells/serum ran through same temps and phases as other tubes.
most often negative |
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Term
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Definition
| after ruling out process only one antigen remains- antibody identified |
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Term
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Definition
after ruling out process multiple antibodies remain; additional testing done on remaining antibodies using additional cells; additional cells must lack one of the antigens but possess the other
3 plus rule |
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Term
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Definition
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