Term
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Definition
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Term
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Definition
| Most nucleated cells of the body |
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Term
True or False:
It is there AGNs recognized by recipient immune system which causes rejection of transplanted organs and tissue |
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Definition
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Term
| HLA AGNs are encoded by what? |
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Definition
| MHC genes on the short arm of Chromosome 6 |
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Term
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Definition
| Major Histocompatibility Complex |
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Term
| HLA AGNs are encoded by the MHC genes on the short arm of what Chromosome? |
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Definition
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Term
| What are the 6 loci of HLA? |
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Definition
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Term
| What loci are considered with Class I AGNs? |
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Definition
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Term
| Class I AGNs help to distinguish between what? |
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Definition
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Term
| Class I AGNs control the activity of what? |
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Definition
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Term
| Controlling the activity of cytolytic T cells is the major mechanism for what? |
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Definition
| Cellular recognition of viral and microbial proteins |
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Term
| What loci are considered with Class II AGNs? |
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Definition
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Term
| Where will you find Class II AGNs? |
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Definition
| Immunity cells such as B cells, activated T cells, and Macrophages |
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Term
| Are AGNs dominant/recessive or co-dominant? |
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Definition
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Term
True or False:
The 6 genes (one @ each locus) will be inherited as a package except for the rare crossover |
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Definition
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Term
| Each inherited chromosome is a ______ |
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Definition
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Term
| The chance that any 2 siblings will have identical haplotypes will be ____ |
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Definition
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Term
| What test can detect patient A,B, C AGNs or ABYs? |
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Definition
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Term
Which type of testing for AGN has the following characteristics:
C' is added - if AGN/ABY reaction has occurred, C' will be activated and destroy the cell.
Cells injured by C' allow a dye to enter and appear opaque. Healthy cells exclude the dye and appear refractive |
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Definition
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Term
Given the Procedure, Name the Test:
1. Donor cells are zapped with mitomycin to prevent cells from proliferating
2. Donor and recipient cells are mixed
3. If cells are not identical for D AGN, recipient's lymphocyte cells will enlargen and their blasts will transform |
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Definition
| Mixed Lymphocyte Culture - D AGN |
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Term
| Mixed Lymphocyte Culture - D AGN, Uptake of a dye is proportional to what? |
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Definition
| Transformation in the dividing cells |
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Term
| What type of testing for AGN is the BEST for BMT to match class II D AGNs? |
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Definition
| Molecular Based Technology |
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Term
| Why is Molecular Based Technology the BEST for BMT to match class II D AGNs? |
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Definition
| PCR amplification that now has allowed HLA detection at the DNA level |
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Term
True or False:
Unless the donor and recipient are genetically identical (twins), foreign antigens on the graft will illicit immune response and cause rejection |
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Definition
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Term
| What organs do you NOT HLA match? |
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Definition
| Liver, Lung, Heart, Pancreas |
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Term
| What organs is the HLA match more important for? |
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Definition
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Term
| ____% survival of HLA matched sibling's kidneys compared to _____% if not matched |
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Definition
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Term
| What can you do to help minimize rejections? |
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Definition
HLA matching
Steroids
Immunosuppressive drugs |
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Term
| Cyclosporin A is a type of what? |
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Definition
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Term
| In Solid Organs, what type of compatibility is more important? |
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Definition
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Term
| For BM or stem cell, you want to match what? |
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Definition
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Term
| For the Liver, Lung, Heart, and Pancreas when transplanting instead of HLA what will you give/use instead? |
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Definition
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Term
| What will cause a patient to start becoming refractory to platelet transfusion treatment? |
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Definition
HLA ABYs
Platelet Specific ABY |
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Term
True or False:
27% of patients will be sensitized after 5 transfusions of platelets |
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Definition
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Term
| What type of donors are best for platelet transfusions? |
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Definition
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Term
True or False:
Platelets have more HLA AGNs than Lymphs |
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Definition
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Term
True or False:
Lymphs have more HLA AGNs than Platelets |
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Definition
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Term
| What can you do to platelets to help prevent sensitization? |
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Definition
| Make platelet transfusions leukocyte poor |
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Term
True or False:
GVHD can be fatal |
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Definition
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Term
| GVHD is a problem in what type of patients? |
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Definition
BMT patients
Lymphopenia patients |
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Term
| What can help to prevent GVHD? |
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Definition
| Pretransfusion Irradiation of units |
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Term
| 1500-5000 rads will render what percent of lymphs incapable of replication? |
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Definition
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Term
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Definition
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Term
| 96% of people with ankylosing spondylitis have what AGN? |
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Definition
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Term
| 100% of people with narcolepsy have what AGN? |
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Definition
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Term
| Can exclude what percent of falsely accused fathers with HLA alone? |
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Definition
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Term
| When HLA is combined with RBC AGNs, what does the % of accuracy go to? |
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Definition
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Term
| What are the 2 types of Non-Immune reactions for the platelet count to not rise as expected after a transfusion? |
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Definition
Clinical conditions
Product Problems |
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Term
| What 2 types of Immune Reaction could happen if the platelet count does not rise as expected after a transfusion? |
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Definition
Alloimmunization to either Class I or II HLA
Alloimmunication to platelet specific AGNs |
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Term
| Describe the Non-Immune Product Problems that are associated with the platelet count not rising after a transfusion |
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Definition
Wrong ABO
Product Plt count too low
Near or at Expiration Date |
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Term
| Describe the Non-Immune Clinical Conditions that are associated with the platelet count not rising after a transfusion |
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Definition
Consumption from DIC or Sepsis
Splenomegaly
Bleeding/Bruising |
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Term
True or False:
Even with a platelet HLA match, there is still a 20-50% failure rate |
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Definition
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Term
True or False:
Single donor is a GOOD way to prevent immunization, but does NOT totally eliminate platelet sensitization |
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Definition
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Term
True or False:
Leukodepleting is GOOD because it appears that the WBCs are MORE likely to cause HLA immunization that the Platelets themselves are |
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Definition
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Term
| With Platelet Crossmatching, what has become the Procedure of Choice? |
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Definition
| Platelet Crossmatch with Solid Phase |
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Term
| With the Platelet Crossmatch Procedure of Choice, the Predictive value of successful transfusions is what? |
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Definition
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Term
| Give the procedure for Capture P / Platelet Cross Match |
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Definition
1. Microtiter plates coated with specific or non-specific platelet ABYs
2. Add donor PRP, centrifuge, Wash out unbound Plts
3. Add LISS & patient serum, incubate
4. Any ABY in the patient's serum that is specific will bind
5. Anti-IgG caoted indicator RBCs are added and centrifuged |
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Term
| What does a + Reaction for Capture P / Platelet Cross Match look like? |
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Definition
No indicator cell button,
because the Anti-IgG coated RBCs attached to the lattice formed by the IgG ABYs in the patient serum & the donor platelets |
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Term
| What does a = Reaction for Capture P / Platelet Cross Match look like? |
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Definition
| No AGN/ABY lattice so indicator cells fall to bottom of well and make a button |
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Term
True or False:
Platelet Cross Match Positive Reaction are the wells that will be used |
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Definition
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Term
| What type of reaction will be used with Capture P / Platelet Cross Match? |
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Definition
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