Term
| State of switch controlling the lambda phage life cycle in the lysogen |
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Definition
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Term
| State of switch controlling the lambda phage life cycle in the lytic cell |
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Definition
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Term
| What does RNA polymerase do on infection? |
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Definition
| It initiates transcription at two promoters, the leftward promoter and rightward promoter |
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Term
| What is the first gene transcribed from the right? |
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Definition
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Term
| What is the first gene transcribed from the left? |
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Definition
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Term
| What type of regulator is transcribed from the N gene? |
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Definition
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Term
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Definition
| Protein N enables RNA polymerase to continue to transcribe through regions of DNA that would otherwise cause transcription to terminate |
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Term
| What is an antiterminator? |
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Definition
| A regulatory proteint that acts by preventing transcription termination |
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Term
| What does CII encode for? |
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Definition
| An activator protein that binds to the Pre (for promoter and repressor establishment) site- this ultimately activates transcription of the c1 gene |
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Term
| What does the C1 gene do? |
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Definition
| The C1 gene encodes for a lambda repressor- this prevents lytic growth |
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Term
| What decides whether a cell will be lysed or lysogenized? |
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Definition
| This is determined by the activity of the CII protein. |
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Term
| Why is the CII protein unstable? |
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Definition
| It is degraded by bacterial proteases that are more active when resources are abundant |
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Term
| Tell me about CII and lambdda repressor when resources are prevalent |
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Definition
| CII is degraded and little lambda repressor is produced- lytic life cycle |
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Term
| Tell me about CII and lambda repressor when resources are scarce |
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Definition
| CII is prevalent and a lot of lambda repressor is produced- lysogenic life cycle |
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Term
| What is int and what activates it? |
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Definition
| Int is an additonal protein required for lysogeny, it is activated by CII, this is an integrase required for integration of the lambda genome into the host chromosome |
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Term
| What is the purpose of CIII? |
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Definition
| It helps shield CII from degradation. Thus, helps promote the lysogenic state. |
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Term
| What happens on infection? |
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Definition
| RNA polymerase transcribes N (leftward) and cro (rightward) genes. |
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Term
| This protein enables transcription of cIII, CII, and other genes |
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Definition
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Term
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Definition
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Term
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Definition
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Term
| Give me a summary of what happens when resources and proteases are abundant... |
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Definition
| cII is degraded, Cro represses CI, the lytic cycle continues |
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Term
| Give me a summary of what happens when resources and proteases are not abundant |
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Definition
| CII is active, CI transcription proceeds at a high level, INT protein integrates the phage chromosome, the CI (lambda repressor) shuts off all genes except itself |
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