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Bacteriology ID / tests
e.g. gram +ve/-ve, rods/cocci, anaerobe, BLA, lactose ferm, haemolytic, cat/oxi etc
28
Veterinary Medicine
Undergraduate 3
06/04/2012

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Term
Enterobacteria - coliforms
Definition
i.e. ferment lactose - red BLA
-E.coli
-Klebsiella
-Enterobacter
Term
Enterobacteria - non-coliforms
Definition
i.e. do not ferment lactose - yellow BLA
-Salmonella
-Shigella
-Proteus
-Yersini
Term
Enterobacteria general
Definition
-gram -ve rods
-cat +ve, oxi -ve
-facultative anaerobes
Term
E.coli
Definition
-a coliform i.e. ferments lactose
-gram -ve rod
-cat +ve, oxi -ve
-facultative anaerobes
-IMViC ++--
-motile
-Urease and H2S -ve
-Lysine decarboxylase +ve
Term
Salmonella
Definition
-a non-coliform i.e. does not ferment lactose
-gram -ve rod
-cat +ve, oxi -ve
-facultative anaerobes
-IMViC -+-+
-motile
-Urease -ve
-H2S and lysine decarboxylase +ve
-need sal-specifc agar and sal-selective broth - would be over-grown by other bact on normal plate
-black centered colonies on XLD agar
Term
E.coli IMViC
Definition
++--
Term
Klebsiella IMViC
Definition
--++
Term
Enterbacter IMViC
Definition
--++
Term
Salmonella IMViC
Definition
-+-+
Term
Pasteurella spp.
Definition
-gram -ve rods / CB
-cat +ve, oxi +ve
-facultative anaerobes
-non-motile
Term
Pasteurella multocida
Definition
-gram -ve rods / CB
-cat +ve, oxi +ve
-facultative anaerobes
-non-motile
-no growth on BLA
-growth on FBA but not haemolytic
-Indole +ve
Term
Pasteurella haemolytica
Definition
-gram -ve rods / CB
-cat +ve, oxi +ve
-facultative anaerobes
-non-motile
-growth on BLA
-growth on FBA and haemolytic
-Indole -ve
Term
Pasteurella multocida and haemolytica differences
Definition
P. multocida does not grow on BLA, P. haemolytica does.
Both grow on FBA: P. multocida not haemolytic, P. haemolytica is
P.multocida indole +ve, P. haemolytica indole -ve
Term
Haemophilus spp.
Definition
-gram -ve rods / CB
-cat ~ oxi ~
-facultative anaerobes
-no growth on BLA
-better growth on HBA c.f. FBA - need X (haemin) +/or V (NAD) (so satellitism around S. aureus)
Term
Pasteurella spp. and haemophilus spp. comparision
Definition
-both gram -ve rods / CB
-both facultative anaerobes
-P. multocida and Haemophilus no growth on BLA, P. haemolytic is growth on BLA
-P. haemolytica and Haemophilus haemolytic
Term
Actinobacillus spp.
Definition
-gram -ve
-facultative anaerobes (aerobic but prefer 10% CO2)
-most grow on BLA (not A. pleuropneuomia)
Term
Bordetella spp.
Definition
gram-ve CB
-cat +ve, oxi +ve
-aerobe
-growth on BLA and FBA
-agglutinate RBCs
Term
Pasterella spp. and Bordetella spp comparison
Definition
-both gram -ve
-P. rod/ CB, B. CB
-both cat+ve, oxi +ve
-P. haemolytica and Bordetella grow on BLA
-All grow on FBA
-P. fac. anaerobes, B. aerobes
Term
Bacteroidaceae
Definition
-gram -ve rods
-strict anaerobes
i.e. special isolation needs
Term
Clostridium spp.
Definition
-gram +ve rods
-SPORE FORMING
-actively motile (except C. perfringens)
Term
Clostridum tetani
Definition
terminal spherical spores
Term
Clostridium perfringens
Definition
-bulls eye haemolysis
-stormy clot formation i.e. lactose fermentation
-Naglar reaction (due to alpha-toxin)
-gelatin liquefaction
NB: not motile c.f. all other Clostridium spp.
Term
Clostridium chauvoei spores
Definition
spores are central and oval - "lemons"
Term
Brucella
Definition
-gram -ve CB (use modified ZN / Machiavello stains)
-cat+ve, oxi +ve (~)
-aerobic but grow better when supplemented w. CO2
-fastidious (grow in blood / serum) - no growth on BLA and slow growing do needs antibiotics
-urease and H2S +ve
-also milk ring test
Term
Campylobacter spp
Definition
gram -ve, curved rods - look like seagulls
-cat +ve, oxi+ve
-microaerophilic (5-10% CO2)
-motile w. polar flagella
Term
Campylobacter fetus - microscopy / culture
Definition
-NOT gram stain
-immunofluorescence
-culture is v. slow growing and difficult to see:
fastidious - requires rich, selective medium
Term
Taylorella equigenitalis
Definition
-gram-ve CB
-cat+ve, oxi +ve
-required HBA amd incr CO2
-non-motile
Term
Bact. associated w. repro tract i.e. Brucella, Campylobacter, Taylorella - comparisons
Definition
-all gram -ve
-B. and T are CB, C. curved rods/seagulls
-all cat +ve, oxi +ve (B. oxi +ve/~)
-all grow better w. / need CO2 supplementation
B. aerobic, better w. CO2
C. microaerophillic w. 5-10% CO2
T. requires incr. CO2
-B. and T. are IC bact.
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