Term
| Most frequently isolated Bacteriodes pathogen from clinical samples: |
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Definition
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Term
| Bacteriodes fragilis characteristics: |
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Definition
| Faint staining GRN. Safety pin shape. Anaerobe. Aerotolerent. Contains functional catalase. |
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Term
|
Definition
| Nonenterotoxigenic Bacteriodes fragilis. |
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Term
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Definition
| Entertoxigenic Bacteriodes fragilis. |
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Term
|
Definition
Significant opportunistic pathogen.
Perforations in the colon can lead to intra-abdominal abscesses.
Bacteremia. |
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Term
|
Definition
Primary pathogen.
Diarrheal disease. |
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Term
| Virulence Factor of NTBF - LPS: |
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Definition
Very low endotoxin activity (1000x less than E. coli)
- because LPS lacks classical O-antigen structures. |
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Term
| Virulence Factor of NTBF - Capsule: |
|
Definition
8 different polysaccharides (PSA - PSH)
Adherence to peritoneal surfaces.
Can induce abscess formation outside colon.
Inhibits phagocytosis.
It is the most complex polysaccharide capsule system. |
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Term
| Virulence Factor of NTBF - Fimbriae: |
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Definition
Binds to:
- Fibrinogen.
- Fibronectin.
- Lactoferrin. |
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Term
| Virulence Factor of NTBF - Protease: |
|
Definition
Degrades complement components.
- similar to S. pyogenes SpeB. |
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Term
| Virulence Factor of ETBF - Bacteriodes fragilis Toxin (BFT): |
|
Definition
AKA - Fragilysin.
- heat-labile zinc metalloprotease.
- cleaves E-cadherin - increases intracellular permeability.
-produces morphological changes in the intestinal epithelium.
- chloride secretion with subsequent fluid loss.
- induces an inflammatory response. |
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Term
| Virulence Factor of ETBF - encoded by the BFT gene - found where? |
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Definition
| Found on conjugative transposon in pathogenicity island (BfPAI) |
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Term
| B. fragilis identification on AnaBAP: |
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Definition
No pigmentation.
No beta hemolysis. |
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Term
| B. fragilis identification on BBE: |
|
Definition
Growth stimulated by bile salts.
Resistant to gentamycin.
Esculin is hydrolyzed. |
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Term
| B. fragilis is highly drug resistant - resistant to: |
|
Definition
Kanamycin.
Vancomycin.
Colistin.
Penicillins (Beta-lactamase producer) |
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Term
| B. fragilis - Glucose fermenter? |
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Definition
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Term
| Second most common anaerobic isolates: |
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Definition
| Prevotella and Porphyromonas. |
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Term
| Prevotella and Porphyromonas are found as normal flora where? |
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Definition
Oral cavity.
Upper and lower GI.
Female uro-genital tract. |
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Term
| Prevotella and Porphyromonas clinical conditions: |
|
Definition
Sinusitis.
Otitis media - Chronic.
Periodontal infections.
Brain abscesses.
Female uro-genital tract infections. |
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Term
| Prevotella and Porphyromonas Gram reactions and morphology: |
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Definition
| Both are Gram Negative coccobacilli. |
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Term
| Prevotella and Porphyromonas produce what kind of protein? |
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Definition
| Proteases that degrade IgG. |
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Term
| Prevotella and Porphyromonas - one ferments glucose, one does not: |
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Definition
Prevotella spp. ferment glucose.
Porphyromonas spp. do not. |
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Term
| Prevotella and Porphyromonas AnaBAP reactions: |
|
Definition
Pigment is produced.
Initially: colonies are tan to buff and fluoresce brick red.
Later: colonies become brown to black.
Often described as having a "foul odor" |
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Term
| Fusobacterium nucleatum is normal flora where? |
|
Definition
Oral cavity - gingival smears.
Upper respiratory tract.
GI tract. |
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Term
| Conditions caused by F. nucleatum: |
|
Definition
Can contribute to periodontitis.
Sinusitis.
Brain abscesses.
Intra-abdominal infections.
Liver abscesses. |
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Term
| Gram stain of F. nucleatum shows: |
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Definition
| Slender GNR with pointed ends. |
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Term
| AnaBAP of F. nucleatum shows: |
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Definition
|
|
Term
| F. nucleatum on Egg Yolk Agar: |
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Definition
| Lipase production may be seen. |
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