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Bacterial Sex Genetics
chapter 3
11
Microbiology
Graduate
12/29/2009

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Term
4 ways in which bacteria exchange genetic material
Definition
1. transformation
2. transduction
3. conjugation
4. transposon insertion
Term
What is "transformation"?
Definition
bacterial cell lysis --> DNA to another bacterial cell --> the recipient bacterial cell takes up the DNA
Term
What is "transduction"?
Definition
Bacteriophage (a virus that infects bacteria) inserts a piece of bacterial DNA to another bacterium
Term
How are "temperate" phages different from "virulent" phages?
Definition
Temperate phages' DNA is incorporated into host bacteria's chromosome
Term
what is "prophage"?
Definition
the integrated temperate phage genome in the bacterial genome
Term
what is "lysogenic"?
Definition
Bacteria that have a prophage integrated into their chromosome
Term
Lysogenic immunity?
Definition
Ability of an integrated bacteriophage (prophage) to block a subsequent infection by a similar bacteriophage.
Term
virulent phages --> ________ transduction
temperate phages --> ________ transduction
Definition
virulent: generalized transduction
temperate: specialized transduction
Term
how does "conjugation" work?
Definition
* self-transmissible plasmid (F plasmid) is transferred through sex pilus to F(-) bacteria
* F(-) bacteria become F(+) bacteria
* major mechanism for transfer of antibiotic resistance
Term
what is a "Hfr cell"?
Definition
a bacterial cell in which F plasmid is incorporated into bacterial genome
Term
What are "transposons"?
Definition
* pieces of DNA that insert themselves into donor chromosome w/o having DNA homology.
* Carry genes for antibiotic resistance and virulence factors.
* donor chromosome examples: of bacteriophage, plasmids, bacterial chromosome
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