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Bacterial Physiology
Bacterial Physiology
42
Microbiology
Professional
03/03/2013

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Term
Autotrophs
Definition
Utilize carbon dioxide as a sole source of carbon
Term
Heterotrophs
Definition

Utilize organic form of carbon 

example: glucose

Term
NH3
Definition
Only form of inorganic that can be directly assembled amino acids
Term
organic nitrogen metabolites
Definition

Glutamate

glutamine

aspartate

asparagine

alanine 

Term
Examples of aerobes
Definition

M. Tuberculosis

bacillus subtilis 

Term
Examples of anaerobes
Definition

C. Botulinum

bacteroides 

Term
Examples of Facultative anerobe
Definition

E. coil

S. aureus

Term
examples of aerotolerant
Definition

S. pneumoniae

S. pyogenes

Term
Examples of microaerophilic
Definition
Campylobacter jejuni
Term
What types of bacteria possess both catalase and SOD
Definition

Aerobe, facultative anaerobes and microaerophlic

microaerophilic have small amounts

Term
What types of bacteria possess neither catalase and SOD
Definition
Anaerobes
Term
What types of bacteria possess only SOD
Definition
Aerotolerant
Term
What types of bacteria grow solely in oxygen
Definition
Aerobe
Term
Grows strictly without oxygen
Definition
Anaerobes
Term
Grows with or without oxygen
Definition
Facultative anaerobes, aerotolerant, micraerophilic
Term
Temperature range of psychrophile
Definition
-5-30 C
Term
Temperature range of mesophile
Definition
10-45 C
Term
Temperature range of thermophile
Definition
25-80 C
Term
Diffusion
Definition
Passive transport, molecules are not moved against a concentration gradient
Term
Facilitated diffusion
Definition
Passive transport, specific membrane proteins allow protein compound to diffuse into the cell
Term
Active transport
Definition
Membrane carrier proteins mediated the transport of substances into cell against their concentration gradient, requires energy
Term
Shock sensitive active transport
Definition

Requires ATP

found in gram negative 

utilize binding proteins found in the periplasm

Term
Shock insensitive
Definition
Does not utilize binding proteins and is coupled to the protonmotive force
Term
Examples of shock sensitive
Definition
Galactose and maltose
Term
Example of shock insensitive
Definition
Lactose
Term
Group transloctation (Phosphotransferase System)
Definition

Used for sugars

sugars are brought into the cell and phosphorylate it at the same time

phosphate donor is PEP

involves at least 4 proteins that act as phosphate carriers of high energy phosphate group from PEP to incoming sugar

saves metabolic eenergy

found in mostly obligate and facultative anaerobes

Term
Which bacteria use PTS
Definition
E. Coli, S. Aureus, and Streptococcus mutans
Term
Which bacteria use Proton Symport (permease)
Definition
E. coli
Term
What sugars does E. Coli?
Definition
Glucose and lactose
Term
What sugar does s.aureus use
Definition
Lactose
Term
What sugar does streptococcus mutans use
Definition
Glucose, mannose, and fructose
Term
Examples of high affinity sidephores that e.coli use
Definition
Enterochelin/enterobactin
Term
Media
Definition
Nutrients that support the growth of bacteria
Term
What are the two types of medium
Definition

Minimal medium (inorganic salts, glucose)

nutrient medium or lb broth

Term
Ways to measure growth
Definition

Measure turbidity (spectrophotometer) 

viable count

determining dry weight, measurement of DNA, RNA, protein

Term
what does growth rate depend on
Definition

Species of bacterium

chemical composition of the medium

temperature 

Term
What are the four phases of bacterial growth
Definition

Lag phase 

exponential or logarithmic phase 

stationary phase

Death phase 

Term
Lag phase
Definition
Variable in length, no cell division
Term
Log phase
Definition
Cells are dividing in a constant rate
Term
Stationary phase
Definition
Decreased growth rate, depletion if nutrients, cell death
Term
Death phase
Definition
Cell death
Term
how do bacteria reproduce
Definition
Binary fission
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