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bacterial growth & metabolism
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23
Microbiology
Undergraduate 3
01/31/2013

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Term
Bacterial Growth
Definition
most-binary fission
-most grow in artificial cultural media
-notable exceptions:
1. only on selective media (legionella)
2. obligate intracelular pathogens (chlamydia)..require tissue or cells
3. require animal models (mycobacterium leprae)
4. have never been grown (treponema pallidum--causes syphillis)
Term
prototroph
Definition
a microorganism that can derive all nutirtional requirements for growth from the micronutrients and macronutrients supplied
Term
fastidious
Definition
microorganisms that have complex and special nutrient requirements
-might require special vitamins or amino acids to grow
Term
atmospheric conditions
Definition
-co2 (may be required as a supplement)
-o2
Term
obligate aerobe
Definition
absolute requirement for o2
Term
microaerophilic
Definition
optimal growth at low o2 pressure
Term
facultative aerobe /
facultative anaerobe
Definition
grow either aerobically or anaerobically
Term
obligate anaerobe
Definition
ideal growth in absence of o2 but may be able to grow in o2
Term
bacterial growth phases
Definition
bacteria grow in four phases but have different requirements in each
1. lag phase ---initial, beginning of growth
2. logarithmic/exponential phase ---great increase in #s
3. stationary phase ---no increase in # of bacteria
4. death ---decline in bacterial #s
Term
bacterial metabolism
Definition
-bacterial cells are highly specialized energy transformers
-metabolism refers to all the biochemical reactions in a cell
-generated by oxidation reactions of substrates (adp--->atp....stores energy for the cell)
-energy---synthesize organic compounds needed by the cell
Term
metabolic classes of bacteria
Definition
1. heterotrophic
-essentially all pathogenic bacteria
-obtain energy from organic compounds
2. photosynthetic
-synthesize their own glucose
3. autotrophic
-no sunlight
-no organic compounds
-use inorganic compounds--ie minerals; co2


***** photosynthetic & autotrophic are less apt to be pathogenic
Term
metabolic energy
Definition
-bacteria may generate energy by:
1. aerobic respiration
2. anaerobic respiration
3. fermentation
4. photosynthesis
Term
aerobic respiration
Definition
1. major energy producing mechanism for aerobes (provides atp + metabolic intermediates)
2. 3 major pathways-linked to one another:
-glycolysis or glycolytic pathway (embden-meyerhof)
-krebs cycle (citrus acid cycle)
-electron transport chain-oxidative phosphorylation.

*for all 3 pathways:
1. glucose=most common substrate (major energy source)
2. bacteria oxidize glucose
3. 1 molecule glucose---up to 38 molecules of atp
Term
glycolytic pathway
Definition
- 1 glucose---> 2 pyruvates
-net gain of +2 atp's
-and NADH generated -converted into more ATP -1 NADH can convert into ~3 ATP's later on
Term
krebs cycle
Definition
1 glucose--> 2 pyruvates
-respirstory process-uses o2
-initial step: pyruvate is decarboxyated to co2 and acetyl coenzyme A
-later portion-cycle: net gain of 2 ATP's and 6 more NADH and 2 FADH2
Term
Electron transport + oxidative phosphorylation
Definition
-series of electron transfers within the cytoplasmic membrane
-generate ADP & ATP
-each NADH=3 ATP's
-each FADH2= 2 ATP's

-accomplished with cytochromes and enzymes
Term
electron transport
Definition
-an electron is transferred from molecule A to molecule B. molecule A is oxidized and molecule B is reduced
Term
metabolic energy
Definition
-bacteria may generate energy by:
1.aerobic respiration
2. anaerobic respiration-final electron acceptor---> not oxygen
3. fermentation
4. photosynthesis
Term
anaerobic respiration
Definition
-utilizes the same pathways as aerobic respiration but:-o2 is not the terminal electron acceptor
instead oxygen containing salts supplied in growth media.
-produce energy by reducing substrate
Term
metabolic energy
Definition
bacteria may generate energy by:
-aerobic respiration
-anaerobic respiration
-fermentation
-photosynthesis
Term
fermentation
Definition
-pasteur-1850's - studies lef to discovery of anaerobic bacteria
-primarily anaerobic:
organic compound (not o2) used as terminal electron acceptor
-produces less energy but supports anaerobic bacterial growth

-generates energy primarily using the glycotic pathway -not the krebs cycle or electron transport chain

-simple organic end-products formed from anaerobic dissimilation or metabolism of glucose
end-products: lactic acid, ethanol, acetic acid, butryic acid
Term
Photosynthesis
Definition
-convert light to ATP
-not a feature of pathogenic bacteria
Term
univesal features of bacterial energy production
Definition
-all major pathways use glucose or hexose catabolism
-chemical energy is conserved by formation of high energy compounds (ADP or ATP)
-preparative steps: key intermediate compounds are formed
-major source of PO4 is ATP/ADP (used for chemical energy)
-for conservation of chemical energy (PO4 is renewed by kinases...results in loss of energy)
-generates:
NADH + H+
NADPH + H+
1 NADH= 3 ATP's
(serve as reducing equivalents. -potential energy generated for each pathway)
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