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most-binary fission -most grow in artificial cultural media -notable exceptions: 1. only on selective media (legionella) 2. obligate intracelular pathogens (chlamydia)..require tissue or cells 3. require animal models (mycobacterium leprae) 4. have never been grown (treponema pallidum--causes syphillis) |
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a microorganism that can derive all nutirtional requirements for growth from the micronutrients and macronutrients supplied |
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microorganisms that have complex and special nutrient requirements -might require special vitamins or amino acids to grow |
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-co2 (may be required as a supplement) -o2 |
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absolute requirement for o2 |
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optimal growth at low o2 pressure |
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facultative aerobe / facultative anaerobe |
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grow either aerobically or anaerobically |
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ideal growth in absence of o2 but may be able to grow in o2 |
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bacteria grow in four phases but have different requirements in each 1. lag phase ---initial, beginning of growth 2. logarithmic/exponential phase ---great increase in #s 3. stationary phase ---no increase in # of bacteria 4. death ---decline in bacterial #s |
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Definition
-bacterial cells are highly specialized energy transformers -metabolism refers to all the biochemical reactions in a cell -generated by oxidation reactions of substrates (adp--->atp....stores energy for the cell) -energy---synthesize organic compounds needed by the cell |
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metabolic classes of bacteria |
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Definition
1. heterotrophic -essentially all pathogenic bacteria -obtain energy from organic compounds 2. photosynthetic -synthesize their own glucose 3. autotrophic -no sunlight -no organic compounds -use inorganic compounds--ie minerals; co2
***** photosynthetic & autotrophic are less apt to be pathogenic |
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Definition
-bacteria may generate energy by: 1. aerobic respiration 2. anaerobic respiration 3. fermentation 4. photosynthesis |
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1. major energy producing mechanism for aerobes (provides atp + metabolic intermediates) 2. 3 major pathways-linked to one another: -glycolysis or glycolytic pathway (embden-meyerhof) -krebs cycle (citrus acid cycle) -electron transport chain-oxidative phosphorylation.
*for all 3 pathways: 1. glucose=most common substrate (major energy source) 2. bacteria oxidize glucose 3. 1 molecule glucose---up to 38 molecules of atp |
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- 1 glucose---> 2 pyruvates -net gain of +2 atp's -and NADH generated -converted into more ATP -1 NADH can convert into ~3 ATP's later on |
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1 glucose--> 2 pyruvates -respirstory process-uses o2 -initial step: pyruvate is decarboxyated to co2 and acetyl coenzyme A -later portion-cycle: net gain of 2 ATP's and 6 more NADH and 2 FADH2 |
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Electron transport + oxidative phosphorylation |
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Definition
-series of electron transfers within the cytoplasmic membrane -generate ADP & ATP -each NADH=3 ATP's -each FADH2= 2 ATP's
-accomplished with cytochromes and enzymes |
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-an electron is transferred from molecule A to molecule B. molecule A is oxidized and molecule B is reduced |
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-bacteria may generate energy by: 1.aerobic respiration 2. anaerobic respiration-final electron acceptor---> not oxygen 3. fermentation 4. photosynthesis |
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-utilizes the same pathways as aerobic respiration but:-o2 is not the terminal electron acceptor instead oxygen containing salts supplied in growth media. -produce energy by reducing substrate |
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bacteria may generate energy by: -aerobic respiration -anaerobic respiration -fermentation -photosynthesis |
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-pasteur-1850's - studies lef to discovery of anaerobic bacteria -primarily anaerobic: organic compound (not o2) used as terminal electron acceptor -produces less energy but supports anaerobic bacterial growth
-generates energy primarily using the glycotic pathway -not the krebs cycle or electron transport chain
-simple organic end-products formed from anaerobic dissimilation or metabolism of glucose end-products: lactic acid, ethanol, acetic acid, butryic acid |
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Definition
-convert light to ATP -not a feature of pathogenic bacteria |
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univesal features of bacterial energy production |
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Definition
-all major pathways use glucose or hexose catabolism -chemical energy is conserved by formation of high energy compounds (ADP or ATP) -preparative steps: key intermediate compounds are formed -major source of PO4 is ATP/ADP (used for chemical energy) -for conservation of chemical energy (PO4 is renewed by kinases...results in loss of energy) -generates: NADH + H+ NADPH + H+ 1 NADH= 3 ATP's (serve as reducing equivalents. -potential energy generated for each pathway) |
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