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Bacteria for Test 2
Living conditions, nutrients, oxygen requirements
35
Microbiology
Undergraduate 2
02/12/2014

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Cards

Term
Why do we need to know about prokaryotes?
Definition

1) They have limited sets of growing conditions

2) They have different nutritional needs

3) They can have medical significance

4) They may have nutritional and industrial uses.

Term
What are the energy requirements for Photoautotrophs?
Definition
Phototrophs obtan energy from sunlight.  Photoautotrophs take sun and CO2 to make organic compounds.  Fix carbon and nitrogen.  (Energy Source: Sun, Carbon Source: CO2)
Term
What are the energy requirements for Photoheterotrophs?
Definition
Photoheterotrophs use the suns energy and take their carbon from organic compounds.  They can be facultative.  Green and Purple bacteria. (Energy: Sun, Carbon source: Organic Compounds)
Term
What are the energy requirements for Chemolithoautrophs?
Definition
Use inorganic compounds for energy.  Gain their carbon from CO2.  Live in sulfur environments or other reduced inorganic compounds.  Primary producers of carbon for their environments.  (Energy Source: Inorganic Chemicals: H2, NH3, NO2-, Fe2+, H2S.  Carbon source: CO2.)
Term
What are the energy requirements for Chemoorganoheterotrophs?
Definition
Use organic compounds for energy and carbon.  (Energy Source: Sugars, amino acids, etc., Carbon Source: Organic Compounds)
Term
What function does an FTS protein perform?
Definition
FTS proteins co-ordinate binary cell division for the microbe.  They tell the cell where to divide by gathering centrally when the cell is ready to divide.
Term
What is a divisome?
Definition
A divisome is where the cell divides.  Location is signaled by clustered FTS proteins.
Term
How do bacteria divide without ther cell structures falling apart?  AKA, Transpeptidation
Definition
The new cell wall structures of Peptidoglycan are inserted into segments of the existing cell during bacterial growth
Term
What are the 5 steps to Microbial fission?
Definition

1) DNA Replication

2) Cell Elongation via Transpeptidation

3) Septum Formation

4) Completion of septum with formation of distinct walls

5) Cell Separation

Term
Why do organisms need a variety of nutrients?
Definition

1) For energy needs

2) To build oganic molecules and cell structures

Term
What are the most common nutrients?
Definition

Carbon (50%)

Nitrogen (14%)

Oxygen (20%)

Hydrogen

Term
What microbe requires Oxygen for a nutrient?
Definition
Obligate Aerobe
Term
What microbe will die if Oxygen is present as a nutrient?
Definition
Obligate Anaerobe
Term
What are the four toxic forms of oxygen?
Definition

1) Singlet Oxygen

2) Superoxide Radical

3) Peroxide

4) Hydroxyl Radical

Term
How do Phototrophs handle Singlet Oxygen?
Definition
Carotenoids remove it.  Usually created during photosynthesis.
Term
How do aerobes handle Superoxide Radicals?
Definition
Superoxide dismutase detoxifies it.  (Enzyme reaction, anaerobics lack this and die in the presence of superoxide radicals)
Term
How do aerobes handle Peroxide?
Definition
Peroxidase and catalase detoxify.  Anaerobes lack these enzymes or have them in very small amounts.
Term
How do aerobes handle Hydroxyl radicals?
Definition
Catalase and Peroxidase enzymes detoxify. (Anaerobes lack these and will die.)
Term
What are the oxygen requirements for Obligate Aerobes?
Definition
Require Oxygen to live.  This is used in their aerobic respiration process.
Term
What are the oxygen requirements for Facultative anaerobes?
Definition
Will grow better with O2 present, however anaerobic is possible if O2 is lacking.  Growth is slower.
Term
What is the oxygen requirement for Obligate Anaerobes?
Definition
Obligate anaerobes can't grow with O2 present.  Brief exposure will kill them.
Term
What is the oxygen requirement for Microaerophiles?
Definition
Require 2-10% Oxygen.  More than 10% will inhibit growth.
Term
What is the oxygen requirement for Aerotolerant microbes?
Definition
They are indifferent.  They can grow with O2 but it's not required for energy.  Fermentation is their only metabolic option.
Term
Limited nitrogen halts what process for microbes?
Definition
Anabolism, or growth for the cell.  Nitrogen is needed for protein and nucleotide formation
Term
What function does Phosphorus serve in microbial growth?
Definition
DNA, RNA, ATP and some proteins, in addtion to the hospholibid membrane.
Term
What function does Sulfur serve in a microbe?
Definition
Amino acid component, helps form tertiary structures in proteins.
Term
What function does Iron serve in microbes?
Definition
Formation of some enzymes
Term
What function does Potassium, Magnesium and Calcium serve in microbes?
Definition
Required for some enzymes.
Term
What 2 things does temperature affect in a microbe?
Definition

1) Protein integrity

2) Lipid membrane

Term
What are the 3 cardinal temperatures?
Definition

1) Minimum

2) Maximum

3) Optimal

Term
What are the 5 temperature groupings for microbes?
Definition

1) Psychrophile

2) Psychtrotroph (not listed in lecture)

3) Mesophile (ideal for humans)

4) Thermophile

5) Hyperthermophile

Term
What PH range do Neutrophiles prefer?
Definition
5-8
Term
What pH range do Acidophiles prefer?
Definition
At or below pH 5.5
Term
What is Carbon Fixation?
Definition
Autotrophs take inorganic carbon and convert it into an organic form.
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