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Antiviral -Immuno Treatment
Antiviral Agents for VCOM 2014
38
Microbiology
Professional
10/21/2010

Additional Microbiology Flashcards

 


 

Cards

Term
Antiviral Agents
Definition

Selectively inhibit viral replication without affecting the normal functions of the host cell. They inhibit the viral nucleic acid replication process. 

 

Remember, there are no broad-specturm antivirals 

Term
Viral infections that have antiviral agents
Definition
Herpesvirus, influenza viruses, hepatitis B and C viruses, respiratory syncytial virus, and HIV
Term
Raltegravir
Definition
binds to the CCR5 coreceptor of some HIV strains
Term
Rimantadine and Amantadine
Definition
bind to the M2 protein of influenza A virus, which inhibit this ion pore in the virion envelope, thus preventing H+ ion influx and uncoating
Term
Enfuvirtide
Definition
inhibits HIV viral fusion protein g 
Term
Ribavirin
Definition

Alters cellular nucleotide pools, inhibits viral RNA synthesis and may cause lethal RNA mutation. 

 

It is used for severe RSV infections and in combination with (interferon) IFV-alpha for chronic hepatitis C virus (HCV) infections 

Term
Analogues of Nucleosides
Definition

prevent DNA chain elongation after recognition of base pairing.

 

These drugs are activated by phosphorylation by cellular or viral kinases. 

 

Examples include many herpes simplex, VZV, cytomeglovirus, and HIV antivirals 

Term
Thymidine analogs
Definition

Inhibit thymine biosynthesis or replace thymidine in DNA which leads to misreading of the DNA and mutations.

 

Examples include: Idoxuridine, Trifluorothymidine, fluorouriacil. 

Term
Foscarnet
Definition
is a nonnucleoside nucleic and polymerase inhibitor that binds to and inhibits the DNA polymerase of all herpesviruses and the reverse transcriptase of HIV.
Term
Nonnucleoside HIV reverse transcriptase inhibitors (DNA from RNA)
Definition
Examples: Vevirapine, delavirdine, efavirenz. These are used in combination therapy with various nucleoside analog inhibitors of that enzyme.
Term
Maraviroc
Definition
inhibits HIV integrase, preventing HIV provirus formation
Term
Protease Inhibitors
Definition

Saquinavir, indinavir, ritonavir, nelfinavir, amprenavir 

 

These inhibit the action of HIV protease; used in combination with AZT and a second nucloside analog as "cocktail" therapy in HIV 

Term
Neuraminidase Inhibitors
Definition

oseltamivir and zanamivir 

 

They inhibit the neuraminidase (essential for viral attachment) of influenza A and B viruses; they may be used for prophylaxis as well as treatment. 

Term
Fomivirsen
Definition

A mRNA Inhibitor. A synthetic oligonucleotide complementary to a sequence in CMV RNA (an antisense compound). It prevents transcription of early CMV genes. 

 

It is approved for intravitreal (inside the eye) therapy of CMV retinitis after other therapies have failed .

Term
Interferons (IFN)
Definition

Are host-coded proteins or glycoproteins produced in and secreted from virus-infected cells in response to viral infection 

 

They are host specific. 

 

Three groups recognized: IFN- alpha, beta, and gamma 

Term
IFN- alpha (Intron - A)
Definition
licensed for treatment of chronic HBV and HCV infections and can produce adverse effects at high doses or with chronic therapy. These side effects include bone marrow suppression.
Term
Laboratory Viral Diagnosis
Definition

Involves: 

1. Virus isolation

2. Direct demonstration of the virus, viral nucleic acid, or antigens in clinical specimens

3. Serologic testing of viral specific antibodies 

Term
Virus Isolation
Definition

Identifies virus replication in susceptible cells . Embryonated eggs or animal hosts often used. 


a. in live infected tissue culture cells, replication may be detected by observing a cytopathogenic effect, polykaryocyte formation or hemadsorption. 

 

b. fixed infected tissue culture cells. Replication may be detected by observing characteristic inclusion bodies (in nucleus or cytoplasm)...deposit of protein or carbs left behind.  

 

 

C. In embryonated egg, replication is detected by pock formation. In animals, by the development of clinical symptoms 

Term
Direct examination of clinical specimens
Definition

samples include tissue biopsies, smears, CSFs, urine, etc

 

Examples of assays: immunohistochemical staining, nucleic acid hybridization, amplification methods

Term
Direct examination of clinical Specimens: Nucleic acid hybridization and amplification
Definition
highly sensitive and specific. detects viral DNA or RNA . PCR may be used to amplify viral genes; Dot Blot hybridization techniques that usually use ss complementary nucleic acid probes
Term
Direct-phase immunoassays: Solid phase Immunoassays
Definition

Detect antigens such as rotavirus and HAV 

 

They use specific viral Abs and radioimmunoassay or enzyme-linked immunosorbent assay. 

 

These assays are highly sensitive and speficic 

Term
Serologic Tests
Definition

Used to determine the titer of specific antiviral Abs. 

 

Paired blood samples are taken. At least a 4fold increase in titer between the samples must be present to indicate a current infection. 

Term
Serologic test: Virus neutralization test
Definition

based on the fact certain Abs will neutralize the cytopathic effect of the virus.

 

If cytopathic effect neutralized by a particular serum: the sample is positive and Abs in the serum were effective against the virus and visaversa. 

Term
Serologic Tests: Hemagglutination inhibition tests
Definition

based on the idea that serum with inhibit viral agglutination of erythrocytes. 

 

Tests can only be done with viruses that have hemagglutinins on their surface (influenza, measles) 

Term
Serologic Test: Solid-phase immunoassays
Definition
Used to detect specific viral Abs. Use RIA and ELISA protocols. several 100xs more sensitive than other serologic tests
Term
Passive Immunization
Definition

injection of human plasma or gamma-globulin fractions from immune individuals 

 

Used to prevent: rubella, measles, mumps, HAV, HBV, rabies, and Varicella-zoster virus (VZV)

Term
DNA vaccines
Definition

Plasmid DNA expressing vectors that elicit both humoral nad cell-mediated immune responses. 

 

Used for HIV and influenza 

Term
Viral polypeptides
Definition

Sequences of virion receptors that have been 

 

Advantages and disadvantages are the same for killed vaccines 

 

Ex: HBV, HEV (hep. E virus) , HPV (human papillomavirus)

Term
Virion Subunit Vaccines
Definition

purified proteins (viral receptors) obtained from virions. 

 

Advantages and disadvantages are the same as for killed vaccines

 

Ex: adenovirus 

Term
Killed Virus Vaccines
Definition

Prepared by heat or chemical inactivation 

 

Advantage: easily combined into vaccines. Doesn't elicit an immune response

 

Disadvantage: lack of development of secretory IgA. You need boosters; poor cell-mediated response

 

Examples: poliovirus, rabies, influenza, HAV

Term
Live Virus Vaccines
Definition

Attenuated strains that are avirulent

 

Advantage: admin. in single dose; induces wide spectrum of Abs and cytotoxic cells 

 

Disadvantages: limited shelf life, possible reversion to virulence, possible production of persistent infection

 

Ex: measles. mumps, rubella, chickenpox, rotavirus, yellow fever, some adenovirus strains

Term
Viral induced Immunopathology
Definition
Rxns (immediate hypersensitivity, antibody-antigen complexes) can contribute to a disease process and are a common feature of persistent viral infections 
Term
Cell-mediated Immunity
Definition

Involves cytotoxic T lymps., Ab-dependent cell-mediated cytotoxicity, NKcells and activated macrophages. 

 

This is the most important defense mechanism against noncytolytic infection (alteration of the virus-infected cell membrane)

Term
Humoral Immunity
Definition
Production of B lymphs. of neutralizing and nonneutralizing Abs against viral-specific antigens. This is the most important against cytolytic viral infections
Term
Neutralizing Abs
Definition

Part of the Humoral Immune system: inhibit the ability of a virus to replicate by inhibiting viral attachment, penetration, uncoating, or all 3 processes .

 

Lesions may also be induced in the viral envelope with the aid of a complement 

Term
Nonneutralizing antibodies
Definition
Part of Humoral immunity: enhance phagocytosis of virions by acting as opsonins.
Term
Interfering RNA (RNAi)
Definition

Process: 

1. ds viral RNA interacts with host ribonuclease DICER

2. Forms short interfering RNAs (siRNAs) which interact with argonaute proteins to form RICS (RNA-inducing silencing complexes)

3. RISC binds to viral mRNA and inhibits viral gene expression 

Term
Nonimmune Defenses
Definition

1. Innate immunity : anatomical and chemical barriers 

2. Cellular resistance

3. Inflammation: creates low pH, elevated temp. 

4. IFN: inhibits viral replication; species specific; the first viral induced defense mech. 

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