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Advanced Biochem midterm
metabolic pathways
35
Biochemistry
Undergraduate 4
03/05/2012

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Term
succinyl CoA
Definition
TCA, fatty acid degradation
Term
oxaloacetate
Definition
TCA, gluconeogenesis
Term
pyruvate fates
Definition
gluconeogenesis, TCA, lactate, oxalacetate, amino acid metabolism, oxidative metabolism, respiration, anaerobic glycolysis, fermentation, recycle cytoplasmic NAD+
Term
NAD/NADH
Definition
glycolysis, PPP, TCA, gluconeogenesis, glycolytic pathway, ketone body metabolism
Term
FAD/FADH2
Definition
TCA(fumerate), e- transport chain(complex III), B oxidation(acyl coa dehydrogenase), oxidative phosphorylation
Term
NADPH
Definition
PPP, photosynthesis, fatty acid activation
Term
succinate
Definition
TCA, e- transport
Term
fumerate
Definition
TCA, Urea
Term
malate
Definition
TCA, red. eq. transport(also uses aspartate)

O=CO-COH-CH2-CO=O

O=CO-CH2-CHNH3-CO=O
Term
substrate level phosphorylations
Definition
glycolysis: phosphoenolpyruvate-->pyruvate
glycolysis: 1,3-bisphosphoglycerate-->3 phosphoglycerate
TCA: succinyl-CoA-->succinate
Term
What occurs in mitochondria?
Definition
TCA, acetyl CoA synthesis, e- trans chain, B oxidation, TAG synthesis(and in ER), Fatty Acid elongation and denaturation(and in ER)
Term
What occurs on the face of the ER?
Definition
phospholipid synthesis
Term
What occurs in the cytoplasm?
Definition
Glycolysis, Fatty Acid synthesis
Term
chylomicrons
Definition
transport exogenous TAG and cholesterol from intestines to tissues
Term
reducing equivalent transport
Definition
NADH in cytosol must be reconverted to NAD+ to maintain glycolysis--uses PEP to travel in and out of mitochondria (also L-malate and L-aspartate)
Term
What are the fates of glucose-6-phosphate?
Definition
glycolysis, PPP, reverse glycogen metabolism
Term
How many ATP are used in the Urea Cycle?
Definition
3
Term
What are the three types of hormones?
Definition
1) diffuse across membranes and interact with steroids
2) can have receptors--in all mammal cells but red blood cells
3) can interact with cell surface receptors(insulin, growth factors, pancreatic islets, g.i. hormones)
Term
What are the four types of receptors?
Definition
1) G-protein coupled(ATP+cAMP=cellular response)
2) ion channel
3) tyrosine kinase (RAS, insulin)
4) intrinsic enzymatic activity (GTP-->cGMP) insulin, growth factors
Term
lipoproteins
Definition
transport TAGs and cholesterol in blood plasma
Term
micelles vs. liposome
Definition
micelles formed by single tail lipids(detergents), liposomes formed by double tailed lipids
Term
e- transport chain
Definition
reoxidizes NADH and FADH2
Term
how can you reduce FAD?
Definition
glycerol-3-phosphate dehydrogenase (also yields 2ATP)
Term
chemiosmotic hypothesis
Definition
coupling synthesis of ATP with e- transport chain
Term
coenzymes of e- transport chain
Definition
FMN/FMNH/FMNH2, coenzyme Q/QH/QH2
Term
How are FAD and NAD different?
Definition
FAD does not pass through complex I and as a result does not produce as many ATPs (2 as opposed to NAD's 3ATP
Term
in the e- transport chain, what is the synthesis of ATP coupled to?
Definition
F1 rotation
Term
A.A.'s are precursors for____?
Definition
Nitrogen containing compounds
Term
compare aerobic and anaerobic
Definition
aerobic: more efficient
anaerobic: faster
Term
end product of pyrimidine synthesis?
Definition
UMP then it can be converted to UTP (both UMP and ATP)
Term
purine synthesis starting and ending points
Definition
starting: ribose-5-phosphate
ending: IMP-->AMP&GMP
Term
how are ketone bodies made?
Definition
acetyl coa (diabetes!)
Term
types of conserved proteins:
Definition
1) GTPase switch(RAS)
2) protein kinases(leads to phosphorylation through activation)
3) adaptor protein(docking)
Term
how does receptor oligomerization activate enzymatic activity?
Definition
1) increases local conc. of PTK, more efficient
2) open configuration, allowing access to ATP and substrates
3) enables phosphotransfer from MgATP tryrosines on receptor itself
Term
PI-3 kinase
Definition
cell survival and apoptosis
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