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Pegnancy Test and blood typing are examples of what type of biotechnology? |
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Fermentation is an example of what type of technology? |
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The technology used to inhibit the production of certain proteins is..... |
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What is genetic engneering? |
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using genetics and traits and applying them to research and development |
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Name an agricultural application of biotechnology |
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An observable form of a trait is called |
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What is Mendels law about different pairs of alleles seperated independent of each other during gamete formation? |
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Name an experiment used to confirm DNA as gentic material |
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Hersey and Chase- they used radio active DNA to show bacterialphage changed genetic material |
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What enzyme is required for transcription? |
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What enzyme is required for cDNA synthesis? |
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What enzyme is required for DNA replication? |
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What enzyme is required for the joining of pieces of nucleic acid? |
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Name two DNA libraries and their difference |
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cDNA (specific, no introns) and genomic (entire) |
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Two differences between PCR and RAPD |
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PCR is general while RAPD is specific |
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a short single strand peice of DNA that is labled and used in hybridization |
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loss or gain of one or more chromosomes |
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loss or gain of entire sets of chromosomes |
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A triploid human will have how many chromosomes? |
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What does VNTR stand for? |
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Variable Number Tandom Repeats |
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What is the name of the transformation technique used to transfor bacteria? |
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What is the name of the medium used to incubate the transformats for one hour? |
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List the steps in DNA cloning |
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Do Like To See Patty 1) Digest 2) Ligate 3) Transform 4) Seperate Cells 5) Purify |
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In Sanger sequencing, what causes DNA sythesis to be terminated at a specfic base? |
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Nucleotide triphosphates that lack oxygen at the 3' position of the sugar |
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What are the steps of PCR? |
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1) Heat to 95-96 (break bonds) 2) Cool to 50-65 (Primers form) 3) Heat to 72 (elongation) |
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What is the function of the enzyme Restriction Endonuclease? |
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The chemical used to visualize DNA under UV light is |
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Name one tool used to transfer DNA |
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Which direction does DNA move during electrophoresis? |
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