Term
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Definition
- substrate/enzyme concentration
- pH, ionic strength
- cofactors, activators, inhibitors |
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Term
Kinetics gives quant understanding of
(4 things) |
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Definition
| - specificity - efficiency of diff enzymes - fx of mutations - enzyme regulation |
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Term
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Definition
- increase velocity
- lowers activation energy
- unconsumed
- active site is region that has substrate binding and catalytic site
- Binding of substrate induces change in enzyme; induced fit model |
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Term
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Definition
- confers specificity in terms of substrate and reaction catalyzed
- determined by tert and possible quat structure |
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Term
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Definition
- Some enzymes require them to fucntion properly
- Are small molecules like lipids and proteins (organic) or Ca, Mg, Fe (inorganic)
- Interact with active site |
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Term
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Definition
- V0 = Vmax [S]/(Km+[S])
- At low [S], V prop to [S]
- At high [S], independant of [S], approaches Vmax |
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Term
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Definition
| - micromole of product/min/mg enzyme |
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Term
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Definition
- All enzyme saturated, change in substrate concentration has no fx
- Measure of how much enzyme is present |
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Term
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Definition
- Michaelis constant
- Measure of affinity; low Km high affinity
- Concentration at which half of active sites filled
- Can vary with conditions |
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Term
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Definition
- Double Recip of MM
- Lower Km = Xintercept closer to origin
- High Vm = Y-intercept closer to origin |
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Term
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Definition
- Binds to active site prevents substrate from binding
- Lowers apparent Km
- Doesnt affect Vmax (increasing substrate will drown out effect"
- ex; HMG CoA reductase is comp inhib by Lovastatin |
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Term
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Definition
- Binds to ES complex
- Decreases Km (lechatlier)
- Decreases vmax because less enzyme availible when locked in substrate position |
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Term
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Definition
- Binds either S or ES
- Doesnt effect affinity (Km)
- Lowers Vmax |
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Term
| Summary 1) Competitive 2) Uncompetitive 3) Noncompetitive |
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Definition
| 1) Effects Slope/X-int, binds E, No fx on VM, increases Km (decreases affinity) 2) Binds ES, Effects intercepts, decreases Vmax and decreases Km 3) Binds E or ES, Effects y-intercept/slope, decreases vmax and doesn't affect Km |
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Term
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Definition
- Dissociation constant of enzyme-inhib
- Ki = [E][I]/[EI]
- Lower Ki = High inhib affinity
- Ki is amnt of inhib needed to reduce enzyme activity by 50% |
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