Term
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Definition
- Removing water in the sample and replacing with soluble solvent with embedding medium
- A harder medium allows the tissue to be sliced thinly |
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Term
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Definition
- Resolution of .2 micrometers
- Fixed in aqueous formaldehyde (proteins and NucAcids)
- Sample is dehydrated thru 50-70-90-100 EtOH washes
- Embedded in paraffin wax
- Slice 5-15 micrometers |
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Term
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Definition
- Fixed in glutaraldehyde then ozmium tetroxide
- Theoretical resolution .05nm, 1nm in actual bio sample
- Dehydratd like LM (using progressive EtOH)
- Embed in epoxy
- Plastics allow 50-150 nm (.05-15um) |
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Term
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Definition
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Term
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Definition
- Basic dye, + charge
- Stains PO4 gprs of NAs, sulfates of glycoaminoglycans, carboxyl groups of proteins; basophiles
- Dark purple |
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Term
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Definition
- Acid dye; - charge
- Binds to cationic components
- Light purple |
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Term
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Definition
- stains carbs; gluco-proteins/lipids/gens
- saints extracell comp like t3 collagen and glycosaminoglycans |
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Term
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Definition
- Metachromatic; colors diff comp diff
- Basic dye
- More bound goes from blue to purple
- ex; Heparin granules of mast cells |
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Term
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Definition
- Raise antibody to an antigen
- Binds to that antigen
- Secondary antibody recognize primary anti-body and ave marker that allow detection
- Common markers; dyes (LM), HRP (L+E/M), gold spheres (EM), alk phosphotase/peroxidase for biochem |
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Term
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Definition
| - 4', 6-diamidino-2 phenylindole; fluorsces blue when bound to DNA |
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Term
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Definition
- Flourescent tag
- Causes translation of tagged PKC
- Allows to track localization of PKC in cell |
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Term
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Definition
- Beads that flouresce red
- Allows to track phagocytosis
- Ex; macrophage |
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Term
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Definition
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Definition
| - http://www.topatoco.com/merchant.mvc?Screen=PROD&Store_Code=TO&Product_Code=NED-MAGIC&Category_Code=NED |
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Term
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Definition
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Term
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Definition
- Junction sites between adjacent cells, darker spots between cells along the surface
- At EM levels it is a complex |
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Term
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Definition
| Green usually (outer most) |
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