Term
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Definition
1) Strucutre with collagen
2) Unique binding sites with IgG
3) Complex structures with little DNA as in viral capsids
4) Molecular machines; ATPsynthase and kinesin |
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Term
| Collagens, Fibroin, and keratins are... |
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Definition
fibrous proteins
- Collagen can make up as much as 3rd of mass of large animal and tensil strength of steel |
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Term
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Definition
- 3 chain triple helix/coiled coil, very tightly packed with small gly at center
Stabilized through hydrophic interactions and hydroxy OH bonds
- Not alpha-helical, GLY-X-Y motif (x-proline, y either prol or hydroxy prol)
- Lysine sometimes subed in for X or Y, allows cross linking |
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Term
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Definition
| - Disrupts non-covalent interactions and crosslinks to form gelatin |
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Term
| _____ catalyzes proline to hydroxyproline |
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Definition
- prolyl hydroxylase
- requires vit c, deficiency leads to scurvy (lesions in skim, gum and vessels) |
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Term
| Genetic Economy and Quat struct |
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Definition
1) Protein can serve as subunit for a large structure
2) Has perks; conserves genetic info, assembly is easily controlled by low energy bonds, synthesis errors are more easily avoided
- ex; Tomato Busy stunt structure made with 180 monomers each made of 386 AA, icosohedral structure made of 3 different "enviroments" patterned, only 4.5k nucleotides |
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Term
| Unique binding/active sites using quat struc |
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Definition
- IgG major serum antibody
- 4 peptide chains; 2 light and 2 heavy
- Each peptide consists of igg domains 2 light and 4 dark
- Domain made of antipar sheets that are stabilized by single disulf bond
- Chains linked thru cov disulf bond between cysteins
- Contain Variable region that make bsite for spec antigen
- IgG domain struct found in many other structsures |
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Term
| IgG has ___ peptide chains; _______ |
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Definition
| 4 peptide chains; 2 light and 2 heavy |
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Term
| Light chain has __ domains and dark chain has ___ domains |
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Definition
| - 2 for light and 4 for dark |
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Term
| Each domain of the IgG is made of... and stabilized by ______ |
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Definition
| - Domain made of antipar sheets that are stabilized by single disulf bond |
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Term
| Chains are linked by .... |
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Definition
| - Chains linked thru covalent disulf bond between cysteins |
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Term
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Definition
- region found in both chains (H/L) that binds to specific antigen
- igg has two such sites
- Allows for greater complementarity |
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Term
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Definition
| - Conserved region between different IgG's |
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Term
| ETC creates a ____ ________ used by ___ _____ to create _____ |
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Definition
| proton gradient, atp synthase, ATP |
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Term
| Structure of ATP Synthase |
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Definition
- Rotary
- Complex heavy protein
- F0 subunit is H+ chanel, moves
- F1 in static, contains catalytic unit where ATP in synth (and orthophosphate) |
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Term
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Definition
| - Transmembrane H+ carrier, consists of 10-15 c subunits, rotate |
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Term
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Definition
- heterohexameric globular a/b structure
- contacts the f0 via gamma stalk/subunit (coiled coil anchor/shaft)
- a/b subunit anchor alpha/beta glob structure
- synthesis occurs on the beta subunit |
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Term
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Definition
| - made of two a-helices with asp acid at the center |
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Term
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Definition
| - two half channels that allow protons to enter and pass partway but not completely |
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Term
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Definition
| 1) Movement of 10 protons is required for 1 ATP |
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Term
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Definition
- proton flow causes gamma sub to rotate
- Each 120* rot causes a change in the conformation of the B subunit into 3 diff forms
- L binds ADP and Pi
- T converts ADP to ATP
- O releases ATP
- 120* corresponds to 1 ATP being synthed |
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Term
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Definition
- Homodimer of 2 nucleotide binding motor domains that are connected through a coiled coil
- N site binds mtube, c transport
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Term
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Definition
- Lagging head is tightly bound to mtubule via atp in binding site while leading head is loosely bound to mtub with adp in binding site
- ADP is exchanged for ATP causing in the lagging strand which changes linker confirmation,
- ATP is added to the leading head which throws lagging head forward due to change linker conformation |
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