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Definition
| The process of generating many identical copies of a recombinant DNA molecule |
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Term
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Definition
- Enzymes that recognize specific DNA sequences and cleave at or near these sequences
- Can make blunt or staggered/sticky cuts
- If EcoR1 cuts at a 6 sequence area, then it cuts once every 4k bases or 4^6 |
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Term
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Definition
DNA molecule into which DNA of interest is inserted for cloning.
Must have:
1) Polylinker
2) Origin of replication
3) Selection trait (amp resistance) |
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Definition
| Organism used to propagate DNA molecule (E. coli or yeast as needed) |
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Definition
| Process of DNA being taken up |
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Definition
| recombinant-containing bacteria |
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Definition
| Act of two complementary single-stranded nucleic acid polymers combining |
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Definition
| a collection of recombinant clones from a given source; 2 subdivisions are cDNA libs which are made from mRNA (thus exons that the sample source actually expresses) and genomic (genomic DNA) |
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Definition
| synthetic DNA made from mRNA using RT |
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Definition
| A filter to which DNA has been transferred after Gel electrophoresis to to seperate DNA by size; Westerns are for protein and Northern for RNA |
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Definition
| Joins two strads of DNA together as long as overhands can basepair |
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Definition
| Insert + Vector; Ligate and transform e. coli; grow in media, distribute on plate and grow (amp plates), trasnfer to filter lyse and hybridize DNA with probe |
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Definition
| DNA Gel -> Souter Blot -> Hybridization |
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Term
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Definition
| Use dideoxy nucleotides to terminate chain elongation. Basically you have DNA in 4 lanes one for each nucleotide with primer + regular nucleotides and dideoxy and DNA pol and let it run so you have various length strands then run the gel. The strands will run differnet lengths allowing you to read the bands along each well for the sequence |
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Term
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Definition
Enzymatic amplification of a DNA fragmentusing primers and heat stable DNA pol
1) Denature (94) seperates the strands 2. Annealing (50-65) allows primers to hybridize to complementary site 3. Extension (72*) to extend primers and generate PCR product |
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