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4.26 Microbiology
By Dr. Wall
28
Pathology
Professional
04/26/2012

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Cards

Term
How long does it take to do an MIC?
Definition
more than 48 hours
Term
Which bacteria would you probably not perform suscetibilities on because they are not really resistant to much?
Definition
beta strep and diptheroids
Term
Green tubes are...
Definition
vacutainers
Term
How should you send tissue to the lab for culture?
Definition
put it in a SMALL amount of saline
Term
How do you collect an anaerobe sample from fluid?
Definition
green top tube
Term
What are acceptable ways of specimen collection to test for anaerobes of the UTI
Definition
bladder tap, supra-pubic aspirate
Term
How do obtain a specimen from the resipratory tract to screen for anaerobes?
Definition
TTA, NO SPUTUM
Term
How do you obtain a vaginal sample if you want to check it for anaerobes?
Definition
endometrial, vag. cuff, placenta
Term
About how long does it take to ID anaerobes and determine susceptbilities?
Definition
4 days to ID; >6 days to determine susceptibility
Term
How should urine be transported?
Definition
vacutainer transport with preservative or sterile jar/tube which needs to be refrigerated
Term
How do you collect blood for culture?
Definition
chlorhexidine for 30 seconds, allow to dry for 30 seconds, 10 mL for each culture (4 mL for pediatric)
Term
A positive blood culture is described as a _____.
Definition
critical value
Term
What is the minimum amount of fluid required to determine bacteria specimen type?
Definition
more than or equal to 1 mL
Term
How do you collect fluid for bacteriology by specimen type?
Definition
sterile jar, green top facutainer (alcohol prep the top), blood culture bottles (but no quantitation or direct smear)
Term
T/F Acid fast culture can be performed on stool.
Definition
false
Term
What is the minimal amount of fluid you need to dtermine specimen type in a respiratory secretion?
Definition
bacteria >1mL, AFB >5mL, fungal >1mL, PCP > 1mL
Term
How should you send a FOBT to the lab?
Definition
inoculate stool directly to the card ASAP for transport to lab because whole blood may degrade rapidly in fresh stool
Term
How do you collect a specimen for C Diff toxin?
Definition
liquid or soft stool, >5 mL, less than 1 hour and do not freeze, no more than 2 bowel movements
Term
How long after a negative C diff test should you wait to retest?
Definition
1 week
Term
How long after a positive C diff test should you wait to repeat testing?
Definition
2 weeks
Term
How do you collect sample for acid fast bacilli?
Definition
3 sputa, > 5 mL, 8 hours apart, 1 early a.m.,
Term
When does the lab stop running AFB tests?
Definition
930
Term
How long does it take to dtermine MTB susceptibility?
Definition
6 weeks
Term
What are the different ways of identifying mTB?
Definition
morphology, HPLC, and gen probe
Term
How should you send a blood specimen for fungal typing?
Definition
blood in the green top
Term
How do you send stool for OAP?
Definition
liquid or soft, > 5 mL, sterile leak proof wide mouthed container, put in fridge
Term
How many OAPs can you do before you have to consult another physician?
Definition
3
Term
How do you collect a sample for PCP?
Definition
> 1mL, put DFA not "silver stain"
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