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303 exam3 lec. 16
oxidative phosphorylation
17
Biochemistry
Undergraduate 3
12/07/2013

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Term
Where does the excess H+ build up vs excess OH-?
Definition
H+ builds up on the cytosol P side
OH on N side= in Matrix
Term
PMF= delta P =
Definition
enerrgy releaed when H+ move across the inner mito membrane from the outside and into the matrix
measured in volts or calories per mole
Term
Complex 1 has =H+ in - = # ATP
Definition
4H+ in intermembrane space= 1ATP
Term
Complex3 has =H+ in - = # ATP
Definition
4H+ in the intermembrane space with in combination of complex 4= 1.5 ATP total
Term
Complex 4 has =H+ in - = # ATP
Definition
2H+ in the intermembrane space with in combination of complex 4= 1.5 ATP total
Term
H+ can re enter the matrix thru
Definition
Fo channel. PMF is catalyzed by F1 complex associated with Fo
Term
ATP synthase rxn:
Definition
ADP +Pi +{H+}out <_> ATP +H2O + {H+}in matrix
Term
What change confirmation of F1
Definition
beta subunit of F1 change confimation as gamma associates with each turn. F1 is matrix side Fo innermembrane side
Term
What has the binding site for ATP
Definition
Beta, gamma associate with one f the 3 alpha/beta pairs forcing the other two nto different confirmation with different nt sites.
Term
The three sites:
Definition
1. beta-ADP has ADP in site
2. the next has ATP in the site bound tightly
3. The 3rd site is empty w/no bound.
Term
Rotation of -- is ==
Definition
gamme is counterclockwise as viewed from matrix side.
Term
What causes ATP to dissociate?
Definition
Confirmation change. B-ADP will then go to B-ATP
Term
ATP wont be released until?
Definition
ADP-Pi are loosely bound in another site.
Term
What causes the electrical and chemical gradient?
Definition
As e- flow in ETC they tak H+ with them across the membrane.
Term
For one turn of F1 there are --H+ flowing thru Fo = +H+ per ATP
Definition
10 H+ per turn
3.3H+ per ATP
Term
p/o ratio=
Definition
number of ATP molecules produces via oxidative phosphorylation per 2e- passing thru ETC
Term
slide 18 very important
Definition
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