Term
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Definition
ds DNA molecule that self replicates independent of chromosomal dna.
smaller, circular, cloning vector, can be EUK |
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Term
| E coli plasmid pBR322 has --bp and -- antibiotic genes |
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Definition
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Term
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Definition
1. cloning vector is cleaved with restriction enzyme
2. dna fragment of interest is cleaved with same enzyme
3. fragments are ligated= recombinant vector
4. introduced to host
5. cloning preduces many copies of recombinant dna |
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Term
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Definition
| pST1 enzyme cleaves in the middle of ampicillan gene= loss of antibiotic resistance to ampillian. You know which bacteria have the new dna if they lose resistance. |
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