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2051
Exam 3
163
Microbiology
Undergraduate 3
11/14/2012

Additional Microbiology Flashcards

 


 

Cards

Term
Catabolism
Definition

breaking down molecules for energy

ex: food is broken down so you can use it for energy

Term
What are catabolic pathways?
Definition

Breakdown large molecules in a series of steps and cause reactions that store energy in small carriers such as

 

ATP and NADH

Term
Anabolism
Definition
using energy to build cell components
Term
Metabolism
Definition

the balance between catabolism and anabolism

 

breaking and building up things

Term

what is used for catabolism and anabolism?

 

Definition
central biochemical pathways (amphibolic)
Term

Whats is the TCA cycle

(Citric Acid or Krebs cycle)?

Definition

glycoliysis, pentose phosphate shunt

chemical reactions used by all aerobic organisms to generate energy through

oxidization of acetate

derived from carbohydrates, fats and proteins

into carbon dioxide.

Term
activation energy
Definition
energy required to bring all molecules in a chemical reaction to a reactive state
Term
enzymes
Definition

catalytic proteins


speed up biochemical reaction rates


lower activation energy by bringing substrates into proximity of each other and correctly orienting them

Term
active/ catalytic site
Definition

portion of an enzyme to which substrate binds


very specific for their substrate

(starch vs cellulose)


they can have small non proteins that help in catlysis but arent part of the enxyme  or substrate

Term

Types of enzymes:

 

Prostethic group-

 

coenzymes-

Definition

prostethic group- bound tightly to their enzyme, usually covalently and permanently

EX:(heme group (non protein) in cytochromes(protein)


Coenzyme- loosely bound to their enzyme, may associate with different enzymes usually derivatives of vitamins

Ex: NAD+ derivative of niacin, can move from one enzyme to another

Term
how are enzymes named?
Definition

named after either the substrate they bind to, or the chemical reaction they catalyze with addition of suffix- ase.

 

ex: cellulase breaks down cellulose into glucose

Term
electron donors:
Definition

lithotrophy- inorganic molecules

 

organotrophy- organic molecules

 

phototrophy- uses light energy to reduce compounds then use these as an electron donor

Term

electron acceptors:

 

Definition

respiration- inorganic molecules

 

fermentation- organic molecules

Term
oxidation reduction reactions:
Definition
involve the transfer of electrons from the electron donor to the electron acceptor
Term
for every oxidation..
Definition
there must also be a reduction
Term

in Redox reaction:

 

OILRIG

Definition

substance oxidized is the electron donor

 

substance reduced in the electron acceptor

Term
electron carrier: for the transfer of electrons fgrom donor to acceptor
Definition

*are membrane bound, prostethic groups like cytochrome c.

 

*freely diffusible coenzymes like NAD+ or NADP+

 

 

Term
NAD+/NADH
Definition
involved in energy generation catabolic reactions
Term
NADP+/NADPH
Definition
involved in biosyntheitc anabolic reactions
Term
NAD+ and NADP+
Definition
act as intermediates between a primary electron donor and a terminal electron acceptor that would normally be redox couples
Term
ATP
Definition

energy released through a redox reaction that is stored in the formaiton of compounds with energy rich bonds

 

main carrier energy of the cell

Term
what does cleavage of each phophohydride bond do?
Definition
releases energy
Term
ATP->ADP->AMP
Definition

phosphate added to ADP via dehydration to make ATP

 

hydrolysis of ATP to ADP yields energy

Term

how does ATP transfer energy to cell processes?

 

Definition
  • hydrolysis, releasing 1-2 phosphates
  • phosphorylation of an organic molecule in the phophotransferase system
  • derivatives of coenzyme A have thioester bonds that release free energy upon hydrolysis
  • long term storage of energy involves glycogen and poly-B-hydrocybutyrate which can be consumed to yeild ATP
Term
Phosphorylation
Definition

 light absorption by chlorophyll drives photolysis of an organic molecule


no exogenous (outside) electron acceptors

electron acceptor must be derived from the electron donor

Term
respiration
Definition

 

(redox)- breakdown of organic molecule with electron transfer to inorganic molecule such as Oxygen or Nitrogen (yeilds more enrgy than fermentation)


molecular oxygen or some other terminal electron acceptor is present


ATP is produced by both substrate level phosphorylation & oxidative phosphorylation

Term
fermentation
Definition

 (redox)- partial breaksdown of organic matter without transfer of electrons to an inorganic terminal electron acceptor


ATP is produced by substrate level phosphorylation during catabolism of an organic compound


substrate level phosphorylation- a phosphate group is added to an intermediate in a biochemical pathway and is eventually transferred from ADP to form ATP

Term
oxidative phosphorylation
Definition

ATP is synthesized by a proton motive force generated by redox reactions

 

requires enzyme ATP synthase which uses proton motive force to produce ATP from ADP.

Term
photophosphorylation
Definition

method of producing ATP in photosynthetic organisms

 

light instead of a chemical compound drives the reox reaction that generates the proton motive force

Term

Bacteria and Archaea

3-Main routes to convert glucose to pyruvate

Definition

glycolysis/Embden-Meyerhof (EMP)

generates 2 ATP and 2 NADH

 

major pathway of glucose metabolism

series of reaction in which each molecule of glucose is oxidized to 2 molecules of pyruvate with a small amount of energy (ATP being generated

 

many carbon molecules are broken down into glucose and then enter glycolysis

Term

Bacteria and Archaea

3-Main routes to convertglucose to pyruvate

Definition

Entner-Doudoroff (ED) pathway

glucose or sugar acids are converted to pyruvate generating 1 ATP, 1 NADH and 1 NADH

 

common in enterics

(intestinal bacteria)


Term

Bacteria and Archaea

3-Main routes to convert glucose to pyruvate

Definition

phentose phosphate shunt (PPS)

 

glucose is converted to sugars with 3 to 7 carbons which are precursors for biosynthesis

 

or to pyruvate generating 1 ATP and 2 NADPH

Term

 

precursor metabolites

(compounds used to make all the macromolecules in the cell)

made in glycolysis

Definition
glucose-6-phosphate
fructose-6-phosphate
glyceraldehyde-3-phosphate
3-phosphoglycerate
phosphoenolpyruvate
pyruvate
Term
Glycolysis summary
Definition
  • glucose is oxidized to 2 molecules of pyruvate
  • net gain of 2 ATP
  • 6 precursor metabolites are made
  • NADH is formed which will be converted back to NAD in the electron transport system or in the fermentation reaction (NADH -> NAD)
Term

Entner-Dudouroff Pathway

 

Definition
  • studied for its role in production of cactus beer by Zymomonas fermentation of the blue agave plant
  • ED pathway allows E. Coli and other enterics to feed on mucus secreted by intestinal epithelium
  • Glucose -> pyruvate (different enzymes than glyco.)
  • Net gain 1 ATP/ glucose and precursor metabolites
  • used by enterics and enterococcus faecalis
Term

Pentose Phosphate Pathway

 

phosphorylations pentose (5 carbon sugars)

Definition

 

 

 

 

formed from G6P

-ribulose
-xylulose
-ribose


used for production of precursor metabolite s in anabolic reactions


Net gain 1 ATP/glucose


 

 respiration-(TCA cycle) to be fully oxidized to carbon dioxide; this makes more ATP, so if an organism has a choice it will choose respiration

 

 

 

 

Term

Ribulose 1,5-biphosphate ( used in Calvin Cycle) is made by phosphorylating a precursor from this pathway

-pyruate can either be used in fermentaion (to make alcohols acids gases)

  respiration-(TCA cycle) to be fully oxidized to carbon dioxide; this makes more ATP, so if an organism has a choice it will choose respiration 

What pathway forms this pyruvate?

Definition
pentose phopshate pathway
Term
fermentation
Definition

can occur in the presence of O2


1 glucose molecule -> 2 ATP


partial oxidation of glucose


reduction of pyruvate


fermentation products

NAD+

Term
respiration
Definition

 

1 glucose molecule -> up to 38 ATP


complete oxidation of glucose to CO2


oxidation of pyruvate by TCA cycle


uses electron transport chain (ETC) and ATP synthesis

 

Term
fermentation
Definition

 

glycolysis occurs followed by fermentation reactions (NO TCA, NO ETC)

  1. electrons from glucose are passed to 2 NAD+ creating NADH
  2. electrons are then passed from NADH to pyruvate, regenerating NAD+ so glycolysis can continue

 

Term
types of fermentation
Definition

homolactic fermentation

 

alcoholic fermentation

Term

homolactic fermentation

 

Definition

once electrons from NADH are passed to pyruvate, pyruvate is reduced to lactic acid

 

ex: lactobacillus- ferments lactose sugar in milk to produce lactic acid, this gives us yogurt

Term
alcoholic fermentation
Definition

once electrons are passed from NADH to pyruvate, pyruvate is reduced to alcohol adn CO2

 

ex: Yeast, ferment the sugars in malted graisn to produce alcohol and CO2 (beer, wine, bread)

Term
In fermentation, who is the elctron donor and who is the elctron acceptor?
Definition

electron donor- glucose

electron acceptor- pyruvate (which is made from glucose)

Term
respiration
Definition

uses oxygen or other compounds from the environment to accept the electrons from NADH

 

Term
aerobic respiration
Definition
uses oxygen to accept electrons
Term
anaerobic respiration
Definition

uses other compounds from the environment other than oxygen

 

may be inorganic compound such as Nitrate, ferric iron, sulfate or carbonate

Term

Tricarboxylic Acid Cycle

 

TCA cycle

krebs cycle

citric acid cycle

 

Definition

cyclic pathway to fully oxidize organic materials

into a small amount of ATP


-NADH and FADH2 (also used in ETC)


-CO2 is a waste product


-Precursor metabolites

Term
TCA Cycle
Definition

 

occurs during respiration


oxidized pyruvate completely to CO2


only possible with an inorganic electron acceptor


produces more energy than fermentation

 

 

Term

pyruvate dehydrogenase complex (PDC)

 

Definition

converts pyruvate to acetyl-CoA


removes CO2 (changes 3 carbons into 2 carbosn) (decarboxylation)

 

 

Term
what is the substrate for the TCA cycle?
Definition
acetyl CoA by way of pyruvate dehydrogenase complex (decarboxylation)
Term
what precursor metabolites are made in the TCA Cycle?
Definition

alpha-ketoglutarate & oxaloacetate

 

(used to make amino acids and nucleotides)

Term
Reactions of the TCA Cycle
Definition

 

 

Acetyl CoA + oxaloacetate (4C)= citrate (6C)


acetyl CoA is oxidized to CO2


original oxaloacetate is regenerated


CO2 is released


NADH & FADH are generated


precursor metabolites are made to be used for biosynthesis

 

 

Term
Aromatic Catabolism
Definition

 

aromatic compounds (benzene rings) converted to pyruvate which enters the TCA cycle


allows for growth in wide range of environments


used for bioremediation

(cleaning oil spills, industrial sites, degrading toxic compounds)

 

Term
aerobic respiration
Definition

 

pair of electrons from NADH is passed through  series of intermediates to oxygen


NADH is oxidized back to NAD+, oxygen is reduced to water


Oxygen is the terminal electron acceptor

 

 

Term
Intermediates that pass electrons from NADH to Oxygen during aerobic respiration
Definition

flavoproteins

cytochromes

quinones

iron-sulfur proteins

Term

ETC

(electron transport chain)

Definition

ETC is made up of intermediates

(such as flavoproteins)


prokaryotes: ETC is the cytoplasmic membrane


eukaryotes: ETC is the mitochondria

(powerhouse of cell)

 

ETC, series of membrane associated electron carriers that carry electrons from the primary electron donor to the terminal electron acceptor

Term
ETC proteins
Definition

 known as oxireducatases-

 

because they oxidize one substrate and thenr educe another

Term
Electron Transport Chain
Definition

different organisms have different ETC

 

some have more than one due to different growth conditions

 

at each step: some electrons are used to push hydogen ion across the cytoplasmic membrane into the periplasm or to the outside of the CM

 

Term

hydrogen ion gradient

proton motive force

chemiosmosis

Definition

creates a hydrogen ion gradient across the CM

The outside of the CM becomes more acidic &

more + charged that the cytoplasm

 

(halophiles use sodium motive forces (Na+ ion gradient)


gradient is a source for potential energy

 

Term

ATPase enzyme

 

ADP -> ATP

Definition

 adds a phosphate group to ADP to make ATP


allows protons to cross back into the cytoplasm


energy is released by reducing the hydrogen ion gradient

this energy is used to make the high energy bond of ATP

oxidative phosphorylation- process of using the hydrogen ion gradient to make ATP

 

Term
high energy phosphate group to ADP to make ATP
Definition
substrate level phosphorylation
Term
energy from hydrogen ion gradient to make ATP
Definition
oxidative phosphorylation
Term
Aerobic respiration summary
Definition

glucose -> pyruvate -> pyruvate is oxidized-> CO2(TCAcycle)



electrons from NADH are transferred to oxygen through ETC

Term
Anaerobic respiration summary
Definition

same as aerobic respiration


energy is used to move protons across cytoplasmic membrane to periplasm

 

ATPase uses energy from hydrogen ion gradient to produce ATP


difference is that the final electron acceptor is some compound other than oxygen

Term
Anabolic Pathways
Definition

11 precursor metabolites in E. Coli

6 from glycolysis 3 from TCA 2 from Pentose phosphate


convert precursors into building blocks (monomers) such as amino acids and nucleotides


Monomers are polymerized to form macromolecules (proteins and nucleic acids) & structures (LPS)

 

Term

Monomer->

Sugar->

Nucleotide->

Amino Acid->

Fatty Acid->

 

Definition

Polymer

Polysaccharide

Nucleic Acid

Protein

Lipid

Term

anabolism-

 

Definition
process by which organisms growing in an environment without building blocks are biosynthesized from simpler components
Term
phototrophs
Definition

use light to form a proton motive force and make ATP

 

cyanobacteria= photoautotrophs

Term
types of photosynthesis
Definition

anoxygenic- no oxygen is produced

(most photosynthetic bacteria are anoxygenic phototrophs)

 

oxygenic- water is split to produce oxygen


cyanobacteria- (only bacterial group) and photosyntheric eukaryotes (plants) are oxygenic autotrophs

Term
pigments of photosynthesis
Definition

chlorophylls in oxygenic phototrophs

 

bacteriochlorophylls in anoxygenic phototrophs

Term
photosynthetic eukaryotes
Definition
have chloroplasts that contain the photosynthetic membranes known as thylakoids
Term
photosynthetic prokaryotes
Definition

do not have chloroplasts


the photosynthetic membranes are:


cytoplasmic membrane in many bacteria


chlorosomes in green bacteria


thylakoid in cyanobacteria

Term
green sulfur bacteria and chlorofexus
Definition
have the ultimate in low light efficiency in regards to photosynthesis in their chlorosomes
Term
antenna chlorophyll molecules
Definition
harvest light energy and transfer it to the reaction center where the conversion of light energy to ATP occurs
Term
wavelengths of light are absorbed...
Definition
differently by different color pigments
Term
absorption spectra
Definition
absorption meter of different chlorophylls and bacteriochlorophylls
Term
cartenoids and phycobilins
Definition
accessory pigments that absorb light and transfer energy to the reaction center chlorophylls
Term
accessory pigments
Definition
allow organisms to capture additional wavelengths of light
Term
cartenoids
Definition
play an important photoprotective role in preventing photooxidative damage to cells (due to toxic forms of oxygen)
Term
anoxygenic photosynthesis
Definition
photosynthesis that does not produce oxygen
Term

anoxygenic phototrophs

 

(use photosynthesis in the light, respiration in the dark and grow in the presence or absence of oxygen)

Definition

 

proteobacteria (purple sulfur and non purple sulfur)


Chloroflexus (green non sulfur)


chlorobium (green sulfur)


heliobacteria (ONLY Gram +)


Rhodobacter species- are used for stufying anoxygenic


photosynthesis

 

 

Term
Oxygenic photosynthesis
Definition

algae & cyanobacteria use electrons from H2O to reduce NADP for CO2 fixation producing O2 as a byproduct


2 seperate reactions :


photosystem I- resembles anoxygenic rxn


photosystem II- splits H2O to yield O2

Term

Autotrophic Fixation

autotrophs use CO2 as sole carbon source

convert CO2 into organic carbon compounds (CO2 fixation)

 

The Calvin Cycle

Definition

RubisCO enzyme catalyzes condensation of CO2 + ribulose,

1-5 biphosphate ->  3- phosphoglycerate -> glyceraldehyde-3-phosphate ->

fructose-6-phosphate


requires large amount of ATP

F-6-P goes into glycolysis

 

Term
carboxysomes
Definition
store molecules of RubisCO
Term
energy demanding process in which CO2 is converted into sugar
Definition
Calvin Cycle
Term

reverse TCA cycle for CO2 fixation

 

uses the hydrooxypropionate pathway

Definition
green sulfur bacteria and chloroflexus
Term
chemolithotrophy
Definition

energy from the oxidation of inorganic electron donors

 

oxidize inroganic chemicals as their sole source of energy

 

autotrophs-> fix CO2 as their carbon source

Term
examples of chemolithotrophy
Definition

 

 

hydrogen oxidation


oxidation of reduced sulfur compounds-(H2S, S)

 

iron oxidation- oxidizes ferroud iron (fe+2)-> ferric iron (fe+3)- most are obligately acidophilic

 

nitrification- convert ammonia to nitrate (NH2->NO3)

 

 

 

Term

nitrate reduction and denitrification

 

Definition

nitrate (NO3-) ->

nitrite (NO2-) ->

(g)Nitric oxide (NO) ->

(g)nitrous oxide (N2O) ->

(g)dinitrogen (N2)

 

all used reductase enzymes

Term
denitrifying bacteria
Definition

reduce nitrate  to gases such as nitrogen, nitrous oxide, nitric oxide

 

nitrate is the electron acceptor


ETC process is the same as aerobic respiration


only difference is that electrons are used to reduce nitrate instead of oxygen

Term
sulfate reduction- sulfate reducing bacteria
Definition
use sulfate as an electron acceptor (anaerobic respiration) converting it to hydrogen sulfide
Term
methanogenesis
Definition
biological production of methane (CH4) from CO2 plus H2 from methylated compoun ds
Term
methanogens
Definition
strictly anaerobic Archaea capable of methanogenesis
Term
nitrogen fixation
Definition

reduction of atmospheric N2 to ammonia NH3

 

energy expensive (40 ATP consumed for fixation of 1 N2)

requires nitrogenase which is inhibited by oxygen


most organisms only fix N when growing anaerobically

 

Term
aerobic nitrogen fixing bacteria protective measures against O2:
Definition

 protective proteins to stabilize

removal of O2 by respiration

O2 retarding slime layers

compartmentalization of nitrogenase in special cells  

(cyanobacteria have heterocysts where N fixation occurs)


some are symbiotic with plants and provide plants the necessary N in exchange for protection (plants grow fuller)

Term
phylogeny
Definition
study of evolutionary relationships of organisms
Term
comparing rRNA sequences is used to...
Definition
determine evolutionary relationships
Term
Ribosomal Database Project
Definition
contains a large collection of rRNA sequences
Term
3 domains of life
Definition

bacteria

archaea

eukarya

Term
Taxonomic Heirarchy
Definition

Domain

Phylum

Class

Order

Family

Genus

Species

Term
binomial system of nomenclature
Definition
descriptive genes and species epithet
Term
international code of nomenclature of bacteria
Definition
regulates naming of prokaryotes
Term
Bergeys Manual of Systematic Bacteriology and The Prokaryotes
Definition
major taxonomic compilations of Bacteria and Archaea
Term
thermophiles
Definition

optimum growth temperatures 80C found near hydrothermal vents and hot springs


fastest growing cells known

 

Term
aquifex
Definition
most ancient and thermophilic of known bacteria grow up to 95 C
Term
thermotoga
Definition
cells surrounded by protein covering (toga) that balloons over ends 
Term
deinococcus
Definition

grouped with thermophiles due to ribosome similarities


highly resistent to radiation due to DNA repair mechanisms


Gram + thick peptidoglycan layer but has outer membrane like G-

Term
thermus aquaticus
Definition

thermophile

DNA polymerase used in PCR

Term
cyanobacteria
Definition

large group of oxygenic phototrophs

fix CO2 (calvin cycle)

fix N2 (heterocysts)

can cause nuisance blooms (takeover water and keep growing)

 have thylakoids- site of photosynthesis

(like chloroplasts)

can grow as:

filaments (many cells growing in a line)

colonies

 

 

Term
cyanobacteria may or may not:
Definition

have gas vesicles for buoyancy


secrete neurotoxins and can kill animals that have ingested the water


form akinetes (specialized spore cells) to survive long periods of dessication and germinate when conditions improve

Term
prochlorophytes
Definition

prochlorococcus

 

possibly the most abundant oxygenic phototroph on earth

Term
Gram + bacteria
Definition

divided into two groups based on % of guanine and cytosine in DNA


low GC- firmicutes

high GC-actinobacteria 

Term
Gram + firmicutes
Definition

rods and cocci

pathogens

staphylococcus


Term

gram +

 

Definition

endospores are extremely heat resistent for millenia

toxin-formers

clostridium (tetanus, botox, gangrene)

bacillus (anthrax, Bt)

Term

molicutes-

ex: mycoplasma

Definition

without a cell wall

small genomes

pleomorphic (no distinct shape)

classifed as Gram + due to phylogenetic relatedness

pathogenic


Term

Gram + Actinobacteria

 

ex: streptomyces (earthy odor of soil)

Definition

branching filaments called mycelia

(like filamentous fungi)

reproductive spores called conidia

sporulation triggered by nutrient depletion

Term

mycobacteria-

 

ex: tuberculosis leprosy

mycobacterium

Definition

acid fast due to mycolic acids

some human pathogens

many are slow growers

 

Term
Gram - Proteobacteria and Nitrospirae
Definition

largest group and most metabolically diverse group of bacteria

5 major phylogenetic subdivisions

Term

alpha proteobacteria-

ex:

rhizobium, argobacterium in plants root nodules plant tumors


rickettsias in animals- rocky mountain spotted fever

Definition

some endosymbionts

get matbolites from host

transmitted between animal arthropods

Term
prostecate/ stalked bacteria
Definition

reproduce by budding

appendages used for attachment

aquatic environments

Term
beta proteobacteria
Definition

lithotrophs- nitrate sulfur iron oxidizers



pathogens- nisseria gonnorrhea Gram - cocci

Term

gamma proteobacteria

 

ex: vibrio and photobacterium

Definition

purple sulfur and non sulfur bacteria

(found in mud and water)

lithotrophs

can use iron or H2S as an electron donor

some are anoxygenic phototrophs

some bioluminescent


Term

enterics

 

ex: e. coli

Definition

colonize human colon


motile- different arrangement of flagella

some in biofilms, some pathogenic

Term

delta proteobacteria

 

myxococcus

Definition

attacks other bacteria in packs, (Social movement)

aggregates into fruiting bodies

disperses myxospores

 

Term

 

delta proteobacteria-

 

bdellovibrio

Definition

parasitizes other bacteria

grows in periplasms

lyse host

Term

epsilon proteobacteria

 

heliobacter pylori

Definition

smallest group of proteobacteria

causes stomach ulcers

burrows below protective mucous layers

Term
nitrospirae
Definition

1st identified by rRNA sequencing (little known about them)


spiral shaped


nitrite oxidizers; obligate aerobes


some found in microbial mats near hot springs

Term

obligate anaerobes

 

bacteroides

Definition

predominant microbe in lower digestive tract of humans and other animals can be pathogenic


in digestive tract, undigested food is fermented by bacteroides


fermentation products are used by animal as carbon and energy source

 

Term

chlorobium 

green sulfur bacterium

Definition

anoxygenic photolithotroph (dont produce oxygen)

contain chlorosomes- bacteriochlorophyll containing structure that are attached to the cytoplasmic membrane

found at greatest depths of water in any phototrophic organism

use H2S as electron donor and oxidize it to sulfur


sulfur granules are deposited outside of the cell

autotrophs- no calvin cycle ; reverse citric acid cycle 

Term
spirochetes
Definition

motile

endoflagella located in the periplasm of the cell



ex:

teronema pallidum causes syphillis

(cant be grown in lab culture)


borrelia burgdorferi causes lyme disease, has a linear chromosome

Term
chlamydia
Definition

obligate intracellular parasites of animals

little metabolic capacity

no peptidoglycan


causes human disease (VD, Psittacosis(epidemic in birds causes pneumonia in humans), conjunctivitis (leads to blindness)


larger reticulate body (grows within cells doesnt survive outside of host)

small elementary bodies (survive outside host, similar in function to endospores)

Term

planctomycetes

 

Gemmata- membrane bound nuclear material (unique in prokaryotes)

Definition

s layer protein cell wall

reproduce by budding

some have stalks for attachment

multiple internal membranes

 

Term

verrucomicrobia-

wrinkled microbes

Definition

irregular shape

contains tubulin

Term
4 phyla of archaea
Definition

Phylum Eukaryarchaeota

Phylum Crenarchaeota

Phylum Nanoarchaeota

Phylum Korararchaeota

Term
Phylum Eukaryarchaeota
Definition

4 groups-extreme halophiles, methanogens, thermoplasmatales, hyperthermophiles


Term

extreme halophiles

 

halobacterium salinarium

Definition

at least 9% NaCl for growth

common in salt lakes and salterns

give red color to water


to prevent water loss in hypertonic environment- 

1) pump inorganic ions (k+) into cell

2) make or concentrate an organic solute in cell

 

Term
methanogens
Definition

release 100 millions tons of methane into atmosphere each year


found in anaerobic environments (strict anaerobes)


freshwater sediments

gastrointestinal tract of animals

rumen

landfills

termite gut

Term

thermoplasmatales

 

thermoplasma

Definition

lack cell walls

 

thermophilic and acidophilic


most strains have been isolated from self heating coal refuse piles

 

Term

hyperhtermophiles

 

pyrococcus "fireball"

Definition
optimum growth at 100 C
Term

Phylum Crenarchaeota

 

sulfolobus

Definition

irregular in shape

may have no cell wall

always have unique liquid

most are hyperthermophiles (hot springs)

some are psychophiles (sea ice)

often very acidic or anaerobic

most use sulfur as electron acceptor

 

Term
Phylum Nanoarchaeota
Definition

nanoarchaeum cells are symbionts or parasites of Ignicoccus (creoarchaeote)

small genome

lack genes for most metabolic functions; depend on the host

hydrothermal vents and hot springs

Term

Phylum Korararchaeota

(Ancient Archael Group)

Definition

found in obsidian pool at yellowstone

hyperthermophiles 85*C

no pure culture exists

Term
Eukaryotic groups
Definition

fungi

algae

protozoa

Term

fungi

 

rhizopus- bread mold

Definition

cell walls contain chitin

non-motile

most grow hyphae

mycelium-branched mass of hyphae

absorptive heterotrophs

 

 

 

Term
chytrids
Definition

 motile flagella reproductive zoospores


(symbiont in bovine rumen, frog pathogen)

Term
yeasts
Definition
unicellular fungi, reproduce via budding
Term
zygomycetes
Definition

nonmotile sporangiospores spread through air or water fuse to form a zygospore


arbuscal mycorrhizae-assoc. w plant roots, increase absorption

Term

ascomycetes

 

candida- yeast that causes thrush and yeast infections

Definition

fruiting bodies form asci containing acsospores

morels and truffles grow low to the grown, very expensive

Term
basidomycetes
Definition

produces spores called basidiospores

 

true mushrooms

Term
Algae
Definition

phytoplankton

all have chloroplasts

many have paired flagella

cell wall made of glycoprotein or cellulose

contractile vacuole removes excess water

stores energy as starch

Term

algae- chlorophyta

 

green algae

Definition

chlorophyll

grow near top of water

multiple life forms

unicellular forms have flagella

filaments- spirogyra

individual cells- chlamydomonas

sheets- ulva

colonies- volvox

Term

algae- rhodophyta

red algae

Definition

phycoerythrin gives red color

sulfated sugar polymers- agar, agarose carrageenan

unicellular, filaments, or sheets

porphya- nori (used for sushi wraps)

Term

Protists- Amoebozoa

 

entamoeba histolytica

Definition

amorphous shape

move using pseupods

actin pushes cytoplasmic streams ahead

cell rolls over membrane

engulf with pseudopods

slime molds

aggregate to form a fruiting body

spores are released from the fruiting body

Term
Protists-Cercozoa
Definition

live in marine habitats


radiolarians- needle-life pseudopods, shells made of silica, stabilized with microtubules


foraminiferans- shells mad eof calcium carbonate, indicators of petroleum deposits

Term
Protists- Alveola
Definition

alveola- flattened vacuoles at outer cortex

extrusomes- secrete enzymes, toxins

microtubules- stabilize the structure

multiple cilia or flagella

Term
alveolates- ciliates
Definition

many cilia for motility, also for feeding

contractile vacuole to maintain osmotic balance

stalked ciliate attach to surface

Term
alveolates- dinoflaggellates
Definition

phytoplankton 

cartenoids in some-red color

two long flagella (one wrapped around cell groove)

extrusome secretes toxins(neurotoxins)

endosymbionts- essential for coral survival

coral bleaching- when dinoflagellates leave the presence of the coral, they will lose their color

Term

alveolates- apicomplexans

 

plasmodium- malaria

Definition

apical complex invades host cell

no cilia

obligate parasite

 

Term

trypanosomes

 

 

Definition

lesihmania major- carried by sand-fly skin and organ infections

 

trypanosoma brucei- carried by tsetse fly(african sleeping sickness)

 

T. cruzi-carried by kissing bug (Chaga's Disease)

Term

excavates

 

Giardia lamblia- passed around in daycares or water (stomach illness)

Definition

lack mitochondria (cant produce energy)


obligate parasites


anaerobes

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