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20 DNA and Chromosomes
powerpoint lecture
24
Biology
Undergraduate 1
03/07/2011

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Cards

Term
DNA is transcribed into ____ and then into protein.
Definition
RNA!!!!
Term
Genome
Definition
an organism's entire set of genetic instructions
Term
proteome
Definition
entire set of proteins that can be made from a genome
Term
Where is DNA found in plant cells and animal cells?
Definition

in plant cells some DNA is in the chloroplasts

In prokaryotes, DNA is not enclosed in the nucleus... it is in contact with the nucleus of the cell

Term
Inner+outer membrane=?
Definition
nuclear envelope
Term
Perinuclear space
Definition
the space between the inner and outer membrane
Term
What membrane is continuous with the rough endoplasmic reticulum?
Definition
the outer membrane
Term
What is continuous with the endoplasmic reticulum lumen ?
Definition
perinuclear space
Term
What are inner and outer membranes fused by?
Definition
nuclear pores
Term

Nuclear lamina proteins 

Definition
– a dynamic structure that form a mesh in the nuclear envelope that keeps the nucleus in tact 
Term
Traffic through pores, what goes in and what goes out?
Definition

In --> proteins (made in the ribosomes which are on the ER) such as histones and enzymes

Out --> ribosomes and mRNA

Term
What type of energy does traffic through nuclear pores use?
Definition
NOT ATP, either CTP, GTP or ITP
Term
Heterochromatin
Definition

dark and dense, found at the centromere or around the edge, will show no gene expression

 

Term
Euchromatin
Definition
lighter and less dense, where you will find genes,  can get into it to express genes
Term
Chromatin
Definition
DNA in it's natural state
Term
DNA in prokaryotes versus eukaryotes
Definition
prokaryotes have a single DNA molecule, eukaryotes have a number of DNA moleucules
Term
Why can't we have gene expression during mitosis?
Definition
because DNA is so tightly packed
Term
Describe bacterial chromosomes
Definition
they are double stranded but circular, they are not packed up super tightly but it is supecoiled which gives it a structure called the nucleoid
Term
How many sets of chromosomes do non-reproductive cells have? What about reproductive ones?
Definition

2n, diploid

n, haploid

Term
Centromere
Definition
where two chromatids are most closely attached ( there are no genes in this region), aren't necessarily in the middle
Term
Histones
Definition
proteins responsible for the first level of DNA packing into chromatin
Term
Nucleosomes
Definition
DNA wrapped around 8 histones, protect DNA around them from enzymes trying to break them down, millions of these but they don't interfere with gene expression, linked together by linker DNA
Term
there are 2 of each histone, what are they called?
Definition
H2A, H2B, H3 and H4
Term
What is chromatin like during interphase?
Definition
the chromatin is loosely packaged to allow for gene expression, and then tightens up just before mitosis 
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